Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis

Pathogens
Yanyan LiuJacqueline M Achkar

Abstract

Sputum smear-negative HIV-associated active tuberculosis (TB) is challenging to diagnose. CD14 is a pattern recognition receptor that is known to mediate monocyte activation. Prior studies have shown increased levels of soluble CD14 (sCD14) as a potential biomarker for TB, but little is known about its value in detecting smear-negative HIV-associated TB. We optimized a sandwich ELISA for the detection of sCD14, and tested sera from 56 smear-negative South African (39 culture-positive and 17 culture-negative) HIV-infected pulmonary TB patients and 24 South African and 43 US (21 positive and 22 negative for tuberculin skin test, respectively) HIV-infected controls. SCD14 concentrations were significantly elevated in smear-negative HIV-associated TB compared with the HIV-infected controls (p< 0.0001), who had similar concentrations, irrespective of the country of origin or the presence or absence of latentM. tuberculosisinfection (p= 0.19). The culture-confirmed TB group had a median sCD14 level of 2199 ng/mL (interquartile range 1927-2719 ng/mL), versus 1148 ng/mL (interquartile range 1053-1412 ng/mL) for the South African controls. At a specificity of 96%, sCD14 had a sensitivity of 95% for culture-confirmed smear-negative TB. T...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
Feb 1, 1966·The Journal of Experimental Medicine·J HurlimannG M Hochwald
Sep 1, 1994·Immunity·J PuginR J Ulevitch
Jun 20, 1997·Science·J M OrensteinS M Wahl
Dec 22, 1999·The Journal of Immunology : Official Journal of the American Association of Immunologists·A KolE A Kurt-Jones
May 23, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·C DempfleR Zerback
Oct 9, 2002·Current Medicinal Chemistry·Katherine Kedzierska, Suzanne M Crowe
May 24, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jae Soon AhnEui Yul Choi
Aug 16, 2003·Science·Brian G Williams, Christopher Dye
Sep 1, 2004·The Journal of Biological Chemistry·Steven BlackDavid Samols
Nov 10, 2005·Journal of Immunological Methods·César Fernández-SánchezVincent Gnanapragasam
Jul 14, 2006·Analytical and Bioanalytical Chemistry·Ryo TanakaEiichi Tamiya
Nov 23, 2006·Nature Medicine·Jason M BrenchleyDaniel C Douek
Aug 5, 2008·Biologicals : Journal of the International Association of Biological Standardization·Peter L Roberts
Dec 17, 2008·Mucosal Immunology·J M Brenchley, D C Douek
Feb 28, 2009·The Lancet Infectious Diseases·Michael J A Reid, N Sarita Shah
Oct 27, 2009·Nature Reviews. Microbiology·Clifton E BarryDouglas Young
Jan 22, 2010·The Cochrane Database of Systematic Reviews·Christopher AkoloJimmy Volmink
Aug 26, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ingrid V BassettKenneth A Freedberg
Jan 22, 2011·The Journal of Infectious Diseases·Netanya G SandlerUNKNOWN INSIGHT SMART Study Group
Feb 5, 2011·Postgraduate Medicine·Elizabeth B WindgassenSharon L Mulvagh
Feb 18, 2011·Nature Reviews. Microbiology·Ruth McNerney, Peter Daley
Oct 26, 2011·The Journal of Infectious Diseases·Jacqueline M Achkar, Elizabeth R Jenny-Avital
Jan 10, 2012·Analytical Chemistry·Vladimir GubalaDavid E Williams
Aug 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rodolphe ThiébautUNKNOWN French ANRS HIV-2 CO5 Cohort
Apr 12, 2013·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·S D LawnR Wood
Feb 11, 2014·The International Journal of Tuberculosis and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·P K DrainD Wilson
Mar 29, 2014·Clinical and Vaccine Immunology : CVI·Michael SievJacqueline M Achkar
May 24, 2014·The International Journal of Biochemistry & Cell Biology·Marianna AgassandianMichael R Shurin
Mar 10, 2015·The Journal of Infectious Diseases·Evguenia KrastinovaUNKNOWN ANRS PRIMO Cohort
Mar 15, 2015·The Journal of Infectious Diseases·Claudia M DenkingerMadhukar Pai
Oct 27, 2015·EBioMedicine·Jacqueline M AchkarEustache Paramithiotis

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleic acid amplification
sandwich immunoassay
X-ray

Software Mentioned

GeneXpert
GraphPad
R
Prism

Related Concepts

Related Feeds