PMID: 7540708Jan 1, 1995Paper

Soluble CD14 in serum mediates LPS-induced increase in permeability of bovine pulmonary arterial endothelial cell monolayers in vitro

Life Sciences
Y IshiiS Kitamura

Abstract

Lipopolysaccharide (LPS) is a mediator of septic shock and acute respiratory distress syndrome (ARDS), conditions which are characterized by high-permeability pulmonary edema. LPS increases endothelial permeability both directly and indirectly via the pro-inflammatory cytokines produced by monocytes and macrophages. We investigated the role of soluble CD14 in serum in the increased endothelial permeability induced by LPS. Bovine pulmonary artery endothelial cells were grown to confluence on a microporous filter and the 125I-albumin clearance rate across the monolayer was determined. Even a high concentration of LPS (1 microgram/ml) did not increase endothelial permeability under a serum-free condition. In the presence of more than 3% normal human serum, LPS increased endothelial permeability. The presence of neutralizing anti-CD14 monoclonal antibody eliminated the serum-dependent effect of LPS. The addition of recombinant sCD14 completely replaced the requirement for serum. LPS-binding protein (LBP) did not enhance the rsCD14-mediated LPS effect, and anti-LBP antibody did not attenuate the serum-dependent LPS effect. These findings suggest that sCD14 in serum mediates the permeability-increasing effect on LPS on endothelial ce...Continue Reading

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Citations

Jan 13, 2000·Clinical and Experimental Immunology·S TakeshitaI Sekine
Aug 31, 2000·Critical Care Medicine·F CarlstedtL Lind
Feb 26, 2005·Infection and Immunity·Samithamby JeyaseelanG Scott Worthen
Mar 18, 2003·American Journal of Respiratory Cell and Molecular Biology·Sadatomo TasakaKazuhiro Yamaguchi
Jun 17, 2011·Journal of Cellular Physiology·Kyung-Mi KimAlexander D Verin
May 19, 2000·The Journal of Surgical Research·C S CoxJ Frederick

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