Soluble CD14 Induces Pro-inflammatory Cytokines in Rheumatoid Arthritis Fibroblast-Like Synovial Cells via Toll-Like Receptor 4.

Cells
Yoshihide IchiseAkio Morinobu

Abstract

Synovial fluids of rheumatoid arthritis (RA) patients commonly contain high concentrations of soluble CD14 (sCD14). To investigate its potential role in RA pathogenesis, we tested whether sCD14 binding transmits a signal to fibroblast-like synoviocytes from RA patients (RA-FLS). The induction of pro-inflammatory cytokines, chemokines, and mediators by sCD14 stimulation of RA-FLS was quantified by real-time PCR and ELISA. Cell proliferation was assessed by the BrdU assay. LPS-RS, a Toll-like receptor 4 (TLR-4) antagonist, was used to block TLR-4 signaling. Soluble CD14 induced the expression of IL-6 mRNA and secretion of the protein. The expression of other pro-inflammatory cytokines and mediators, such as TNF-α, IL-8, intercellular adhesion molecule-1 (ICAM-1), MMP-3, and RANK ligand (RANKL), was also induced by sCD14. In addition, sCD14 stimulation promoted RA-FLS proliferation. LPS-RS abolished IL-6, IL-8, and ICAM-1 mRNA induction by sCD14 in RA-FLS. On the other hand, TNF-α and IL-17A increased TLR-4 expression by RA-FLS and amplified their sCD14-induced IL-6 expression. Soluble CD14 transmits inflammatory signals to RA-FLS via TLR-4. The effects of sCD14 may be augmented in inflammatory milieu. Our results suggest that sCD...Continue Reading

References

Apr 1, 1995·Immunopharmacology·T L Kielian, F Blecha
Jun 1, 1994·Arthritis and Rheumatism·N J Zvaifler, G S Firestein
Aug 30, 2000·Nature·A Aderem, R J Ulevitch
Sep 19, 2002·Cardiovascular Research·Stefanie ZeukeHolger Heine
May 16, 2003·Nature·Gary S Firestein
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sylvette BasCem Gabay
May 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefan DunzendorferPeter S Tobias
Jan 7, 2011·Journal of Periodontal Research·K FeghaliD Grenier
Nov 14, 2012·Nature Reviews. Rheumatology·Nunzio Bottini, Gary S Firestein
Feb 14, 2014·BioMed Research International·Yuji Yoshida, Toshio Tanaka
Sep 16, 2014·Journal of Proteome Research·Min Jueng KangWan-Uk Kim
Jun 4, 2015·AIDS·Carey L ShiveMichael M Lederman
Jan 27, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Manuella LévêqueCorinne Martin-Chouly
Jun 6, 2018·Frontiers in Physiology·Ana Alonso-PérezRodolfo Gómez
Jan 25, 2020·Arthritis Research & Therapy·Snigdha SamarpitaKyoung Soo Kim

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Citations

Jun 8, 2021·Immunological Reviews·Lucy-Jayne MarshAdam P Croft

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Methods Mentioned

BETA
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