Soluble CD14 mediates lipopolysaccharide-induced intercellular adhesion molecule 1 expression in cultured human gingival fibroblasts.

Infection and Immunity
J HayashiI Ishikawa

Abstract

Intercellular adhesion molecule 1 (ICAM-1) is involved in the accumulation and activation of leukocytes in inflammatory diseases such as periodontitis. As reported previously, ICAM-1 is up-regulated on cultured human gingival fibroblasts (HGF) by exposure to lipopolysaccharide (LPS), suggesting a specific LPS recognition mechanism. We therefore investigated the role of CD14, an LPS receptor, in stimulation of HGF by LPS. Cell surface CD14 antigen was not observed on HGF by flow cytometric analysis. In addition, expression of CD14 mRNA in HGF was not detected by reverse transcription-PCR analysis. Since HGF did not express endogenous CD14, we investigated the role of human serum-derived soluble CD14 (sCD14) in ICAM-1 induction on HGF by LPS. The serum-dependent ICAM-1 induction by LPS was observed in HGF. In medium containing human serum, anti-CD14 antibody inhibited ICAM-1 induction on HGF by LPS. Depletion of sCD14 from human serum markedly reduced ICAM-1 expression on HGF in response to LPS. Supplementation of the serum-free medium with sCD14 alone restored the capacity of HGF to respond to LPS. These results show that induction of ICAM-1 in HGF by LPS does not involve binding to cell surface CD14 but is mediated by serum-der...Continue Reading

References

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Citations

Mar 24, 2016·Japanese Journal of Infectious Diseases·Özlem DemirpençeMehmet Bakır
Mar 1, 2006·European Journal of Immunology·Michael HuberMarina A Freudenberg
Aug 16, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Simona B NeffBeatrice Beck-Schimmer
May 1, 2001·Journal of Periodontology·Lijian Jin, Richard P Darveau
Oct 23, 2010·Journal of Clinical Periodontology·Irundika H K DiasHelen R Griffiths
Dec 24, 1998·Infection and Immunity·J HayashiI Ishikawa
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P-L Wang, K Ohura

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