Soluble chemokine CCR5 receptor is present in human plasma

Immunology Letters
Alexander TsimanisZvi Bentwich

Abstract

In view of the natural resistance to infection by HIV and occasional delayed clinical manifestation of the disease, as also the fact that the virus is able to enter only cells that express CD4 and a co-receptor, we initiated a search for a soluble co-receptor that might compete with its membrane counterpart. Using a sandwich ELISA system, a soluble human CCR5 receptor (sCCR5) was indeed detected in the circulation. Immunoprecipitation of sCCR5-positive plasma samples from Israelis of Ethiopian and non-Ethiopian origin with mAb 2D7, a conformation-dependent anti-CCR5 antibody, revealed the presence of an approximately 22 kDa protein. A panel of antibodies directed against the membrane receptor was used to characterize the structure of the soluble CCR5: mAb CTC8, recognizing the N-terminal sequence of the protein, 10YDIN13; "multidomain" mAbs FAB181B and FAB183B that are dependent upon the presence of Q93 and D95 in ECL1 and K171 and E172 in ECL2A, and mAb FAB182B, recognizing the stretch 184YSQYQF189, which spans the C-terminal part of the second extracellular loop. The presence of short soluble CCR5 in human plasma has not been previously described. Among HIV-negative non-Ethiopian Israelis, 20.4% were sCCR5-positive, as agains...Continue Reading

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Citations

Aug 7, 2007·AIDS Research and Human Retroviruses·A BritoJ Casseb
Mar 29, 2011·Analytical Biochemistry·Etienne MalvoisinNadine Vincent
Jun 11, 2010·Indian Journal of Nephrology·M PrakashM Supriya
Sep 2, 2020·International Journal of Molecular Sciences·Ala Litman-ZawadzkaBarbara Mroczko
Aug 29, 2018·BioMed Research International·Emilia LubowickaSławomir Ławicki
Oct 20, 2020·Frontiers in Public Health·Mark Christopher Arokiaraj
Jun 2, 2020·Virus Research·Joel Henrique EllwangerJosé Artur Bogo Chies

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