Soluble enzyme cross-linking via multi-component reactions: a new generation of cross-linked enzymes.

Chemical Communications : Chem Comm
Maryam AshjariMehdi Mohammadi

Abstract

By using a isocyanide-based multi-component reaction for the immobilization of the soluble forms of Rhizomucor miehei lipase (RML) and Thermomyces lanuginosa lipase (TLL), the first step of enzyme aggregation or crystallization in the traditional methods of cross-linking was bypassed. High immobilization yields and specific activities were achieved for both lipases.

References

Sep 1, 1987·Applied Biochemistry and Biotechnology·S K Khare, M N Gupta
Mar 1, 1967·Archives of Biochemistry and Biophysics·A F Habeeb
May 29, 2000·Current Opinion in Chemical Biology·A Dömling
Feb 24, 2001·Nature·A M Klibanov
Aug 29, 2003·Current Opinion in Biotechnology·Linqiu CaoRoger A Sheldon
Aug 27, 2004·Biotechnology and Bioengineering·R SchoevaartR A Sheldon
Feb 28, 2006·Analytical Biochemistry·Shweta ShahMunishwar N Gupta
Nov 23, 2007·Biochemical Society Transactions·C MateoJ M Guisán
Mar 28, 2013·Chemical Society Reviews·Roger A Sheldon, Sander van Pelt
Mar 15, 2015·Enzyme and Microbial Technology·Evelin A ManoelRoberto Fernandez-Lafuente
Apr 26, 2018·Biotechnology Progress·Margarita D RamosPaulo W Tardioli
Aug 21, 2018·International Journal of Biological Macromolecules·Fatemeh SalamiMehdi Mohammadi
Dec 23, 2019·Current Opinion in Chemical Biology·María Romero-Fernández, Francesca Paradisi
Jan 17, 2020·ACS Applied Materials & Interfaces·Mahbuba Jannat, Kun-Lin Yang
Jan 28, 2020·Journal of the American Chemical Society·James S WeltzJoel L Kaar

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.