Soluble factors from human endometrium promote angiogenesis and regulate the endothelial cell transcriptome

Human Reproduction
Cristin PrintStephen Smith

Abstract

Angiogenesis and vascular remodeling play critical roles in the cyclical growth and regression of endometrium. They also appear to play roles in the pathogenesis of endometriosis. Supernatants were collected from cultured endometrium isolated from women with and without endometriosis. These supernatants induced endothelial cell proliferation and angiogenesis in vitro. They contained vascular endothelial growth factor (VEGF)-A, and their proliferative effects on endothelial cells were partially abrogated by a blocking anti-VEGF-A antibody. Gene array analysis showed that culture supernatants from proliferative phase endometrium, and to a lesser extent secretory phase endometrium, induced significant changes in the transcriptome of endothelial cells. We could not detect any association between endometriosis and the ability of endometrial-derived soluble factors to promote angiogenesis or to regulate the endothelial transcriptome. In addition, we could not detect any association between endometriosis and the concentration of VEGF-A in supernatants from cultured endometrium or in menstrual effluent. We have shown that endometrium cultured in vitro produced soluble factors, including VEGF-A, that promoted angiogenesis. Proliferative...Continue Reading

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Citations

Oct 8, 2005·Angiogenesis·P G GroothuisR Grümmer
Oct 8, 2005·Angiogenesis·Jane E Girling, Peter A W Rogers
Jul 25, 2008·Molecular Human Reproduction·Zhen Zhen ZhaoGrant W Montgomery
Sep 15, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cláudia Maria da SilvaMárcia Mendonça Carneiro
Aug 22, 2008·Immunological Investigations·Caroline DunkRebecca Lee Jones
Apr 22, 2011·Reproductive Biomedicine Online·I VerginadisK Charalampopoulos
Feb 16, 2006·Reproductive Biomedicine Online·Shazia MalikStephen K Smith
Dec 24, 2017·Journal of Molecular Endocrinology·Kanchan GuptaAnila Dwivedi
Jul 13, 2006·Human Reproduction Update·Thomas StrowitzkiM von Wolff
Feb 2, 2021·Journal, Genetic Engineering & Biotechnology·Sunil AbrahamAmutha Chinnaiah
May 27, 2021·Clinical Epigenetics·Alejandra Monserrat Retis-ResendizEdgar Ricardo Vázquez-Martínez

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