Soluble Fas ligand in the joints of patients with rheumatoid arthritis and osteoarthritis

Arthritis and Rheumatism
H HashimotoT Ochi

Abstract

To investigate the expression and function of Fas ligand (FasL),which can be in a membrane-bound or soluble form, in the joints of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). The concentration of soluble FasL (sFasL) in serum and synovial fluid (SF) from 24 OA and 38 RA patients was measured using an enzyme-linked immunosorbent assay. The expression of FasL on SF lymphocytes (SFL) and peripheral blood lymphocytes (PBL) was assessed by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. A cytotoxic killing assay of membrane-bound FasL and purified sFasL against cultured synovial cells was also performed. Soluble FasL was detected in the SF of patients with RA and OA, but not in their serum. The concentration of SF sFasL was remarkably higher in patients with severe RA than in patients with mild RA or with OA. RT-PCR showed that SFL, but not PBL, from RA patients expressed messenger RNA for FasL. Membrane-bound FasL induced apoptosis in cultured synovial cells from the RA and OA patients, but naturally processed human sFasL did not. SFL from RA patients expressed FasL, and cleaved sFasL accumulated in the SF of inflamed joints. The different killing activity of membrane-bound FasL and sFasL aga...Continue Reading

References

Jan 1, 1989·Rheumatology International·S Gay, R E Gay
Mar 10, 1995·Science·S Nagata, P Golstein
Apr 1, 1995·Arthritis and Rheumatism·T NakajimaK Nishioka
Sep 1, 1995·The Journal of Clinical Investigation·G S FiresteinN J Zvaifler
Oct 1, 1994·Clinical Immunology and Immunopathology·J A KummerF C Breedveld
Dec 1, 1993·Annals of the Rheumatic Diseases·M N FarahatG S Panayi
Mar 1, 1996·Nature Medicine·M TanakaS Nagata
Jul 15, 1996·The Journal of Clinical Investigation·K FujisawaK Nishioka

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Citations

Oct 18, 2003·Seminars in Immunology·Bryan C BarnhartMarcus E Peter
Feb 13, 2001·Drug Discovery Today·M E. NuttallJ A. Erhardt
Jun 24, 2000·Bone·J T BettanyP S Grabowski
Jul 4, 2013·Investigative Ophthalmology & Visual Science·Hui ZhaoThomas A Ferguson
Apr 27, 2007·Arthritis Research & Therapy·Wan-Uk KimChul-Soo Cho
Nov 24, 2011·Clinical & Developmental Immunology·Boel De PaepeJan L De Bleecker
Oct 30, 2008·Journal of Immunotoxicology·Sarah J BlossomKathleen M Gilbert
Sep 4, 2015·International Journal of Molecular Sciences·Giuseppe MusumeciAli Mobasheri
Apr 28, 2006·Clinical Lymphoma & Myeloma·David GhezOlivier Hermine
Dec 8, 2014·Cytokine·Flavia Calmon-HamatyMichael Hahne
Sep 20, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·María José Martínez-LorenzoLuis Larrad
Jul 21, 2004·Rheumatic Diseases Clinics of North America·Hongtao Liu, Richard M Pope
Dec 22, 2016·International Journal of Molecular Sciences·Edith CharlierDominique De Seny
Nov 20, 2016·The FEBS Journal·Daniela SiegmundHarald Wajant
May 24, 2005·Biochimica Et Biophysica Acta·Marcus E PeterBryan C Barnhart
Oct 17, 2017·The FEBS Journal·Jean-Philippe Guégan, Patrick Legembre
Sep 5, 2002·Arthritis and Rheumatism·T Aigner, H A Kim
May 18, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hideaki MurakamiKoichi Nemoto
Mar 17, 2012·The Journal of Rheumatology·Dimitrios Makrygiannakis, Anca Irinel Catrina
Feb 1, 2007·Arthritis and Rheumatism·Lazaros I Sakkas, Chris D Platsoucas
Aug 11, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M E NuttallM W Lark
Aug 24, 2016·Nature Reviews. Rheumatology·Carla M CudaHarris Perlman
Nov 2, 2011·Cellular and Molecular Life Sciences : CMLS·Sébastien TauzinPatrick Legembre
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K HayashidaP E Lipsky
May 24, 2017·Journal of Physical Therapy Science·Hideaki TakahashiHideaki Onishi
Dec 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nathaniel J BrownHarris Perlman

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