Soluble fibrin augments spreading of fibroblasts by providing RGD sequences of fibrinogen in soluble fibrin

Thrombosis Research
Wei YangM Matsuda

Abstract

We previously reported that fibroblasts were found to spread far more avidly on NaBr-solubilized fibrin monomer (FM) monolayers than on immobilized fibrinogen (Fbg), indicating that removal of fibrinopeptides by thrombin is a prerequisite for the fibrin-mediated augmentation of cell spreading [J. Biol. Chem. 272 (1997) 8824-8829]. Soluble fibrin (SF), a 1:2 complex of fibrin-monomer and fibrinogen, is known to be present in the circulating blood under the pathological condition in which blood coagulation is activated. However, its physiological roles are still incompletely known. Fibroblasts spread on immobilized purified soluble fibrin. Cells spreading on immobilized soluble fibrin were blocked by the exogenous addition of soluble fibrin and glycine-arginine-glycine-aspartic acid-serine-phenylalanine (GRGDSP)-synthetic peptide but not by the addition of fibrinogen or fibrin monomer. However, cell spreading activity was decreased in the surfaces coated with fragment X, whose Aalpha-chains lack carboxyl-terminal segments including arginine-glycine-aspartic acid (RGD)-2 domain, fibrin monomer complexes. It suggests that the RGD-2 domain of fibrinogen after being complexed with fibrin monomer plays a pivotal role for soluble fibri...Continue Reading

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Citations

May 1, 2006·Future Cardiology·Panayiotis G Vlachoyiannopoulos, Michael Samarkos
Feb 7, 2015·Biotechnology and Bioengineering·Laura R GeussLaura J Suggs
Jun 21, 2014·Tissue Engineering. Part B, Reviews·Matthew RiopelRennian Wang
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Apr 28, 2006·Biopolymers·Lilia A ChtcheglovaAndré Haeberli
Jun 9, 2016·Tissue Engineering. Part B, Reviews·Maribella DomenechDonald O Freytes

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