Soluble glycosylated phosphoproteins of cementum

Calcified Tissue International
M J Glimcher, B Lefteriou

Abstract

Ethylenediaminetetraacetate and hydrochloric acid (EDTA) (HCl) extracts of cementum were fractionated by molecular sieving, ion exchange chromotography, and reverse phase high precision liquid chromatography (HPLC). Nine fractions were isolated, all of which contained serine phosphate, threonine phosphate, and high concentrations of aspartic acid (asp) and glutamic acid (glu). Five of the fractions obtained by repeated HPLC consisted of a single band by SDS-PAGE; the others contained at least one other minor component. All of the protein bands stained with both Rhodamine B and alcian blue, the latter consistent with analytical determinations that demonstrated that the phosphoprotein component contained a significant amount of carbohydrate, including neuraminic acid.

References

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Citations

Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A L BronckersW T Butler
Jul 1, 1992·Bone and Mineral·H ArzateA S Narayanan
Mar 7, 2017·Journal of Periodontal Research·B L Foster

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