Soluble interleukin-6 receptor strongly increases the production of acute-phase protein by hepatoma cells but exerts minimal changes on human primary hepatocytes

European Journal of Immunology
C GabayP A Guerne

Abstract

Interleukin-6 (IL-6) interacts with a system of receptors, which include a 80-kDa IL-6-binding subunit (IL-6R) and a transducing element (gp130). The soluble form of IL-6R (sIL-6R) can bind its ligand and induce cellular responses by association with gp130, thus acting as an IL-6 agonist. We and others have previously shown that the responsiveness to IL-6 is different in hepatoma and human primary hepatocytes. We therefore compared the effects of sIL-6R on the two types of cells, and on the B9 hybridoma, another IL-6-sensitive cell line. Human primary hepatocytes, hepatoma cells PLC/PRF/5, and B9 cells were incubated with different concentrations of IL-6, sIL-6-R, or both. The hepatocyte culture supernatants were tested for their content of acute-phase proteins (APP). The proliferation of B9 cells was assessed by a colorimetric method. Results showed that sIL-6R alone markedly increased the production of APP by hepatoma cells in a dose-dependent manner, but affects only minimally primary hepatocytes and the proliferation of B9 cells. The combinations of IL-6R and its ligand enhanced the effects of Il-6 alone in both PLC/PRF/5 and B9 cells, but had no effect on primary hepatocytes. An immunohistochemical study indicated that the...Continue Reading

References

Jan 1, 1976·Methods in Cell Biology·P O Seglen
Oct 23, 1992·Science·T KishimotoT Taga
Sep 13, 1991·Journal of Immunological Methods·N W RoehmA L Glasebrook
May 31, 1990·European Journal of Biochemistry·S Rose-JohnP C Heinrich
Nov 25, 1988·Nucleic Acids Research·M Wilkinson
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R M GrossmanA B Gottlieb
Oct 1, 1989·The Journal of Experimental Medicine·D NovickM Rubinstein
Aug 1, 1994·Journal of Immunological Methods·M DiamantK Bendtzen
Jan 1, 1993·Advances in Immunology·S AkiraT Kishimoto
Feb 1, 1993·European Journal of Immunology·J MüllbergS Rose-John

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Citations

Mar 1, 1997·The Journal of Pathology·H Moshage
Sep 9, 1996·Journal of Immunological Methods·B OppmannS Rose-John
Jan 9, 2013·The Journal of Biological Chemistry·Sun Wook ChoLaurie K McCauley
Jun 15, 1997·The Journal of Clinical Investigation·C GabayW P Arend
Jun 19, 2008·Arthritis Research & Therapy·Josef S SmolenOswald Wagner
Mar 1, 2000·Journal of Neuroimmunology·N J Van Wagoner, E N Benveniste
Sep 13, 2005·Developmental and Comparative Immunology·Norihisa NishimichiHaruo Matsuda
Jul 9, 2008·Scandinavian Journal of Gastroenterology·Othmar LudwiczekHerbert Tilg
Jan 8, 2021·Clinical Oral Investigations·C SchweitzerMarcela Hernández Rios

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