PMID: 375229Mar 1, 1979Paper

Soluble precursor of an integral membrane protein: synthesis of procoat protein in Escherichia coli infected with bacteriophage M13

Proceedings of the National Academy of Sciences of the United States of America
K ItoW Wickner

Abstract

Prior to virus assembly, the major coat protein of coliphage M13 is an integral protein of the host cytoplasmic membrane. Coat protein synthesized in vitro is initially made with an NH2-terminal "leader peptide" of 23 amino acids and is termed "procoat." We now report that procoat is a biosynthetic precursor of coat protein in vivo. Conversion of procoat to coat occurs within 30 sec in cells infected with wild-type virus. This proteolytic processing is delayed in cells infected by M13 mutants (in genes 1, 5, or 7) that are defective in virus assembly. Pulse--chase experiments in combination with subcellular fractionation show that procoat is synthesized in a soluble form in the cytoplasm and is then incorporated into the cytoplasmic membrane, where it is converted to coat protein. This finding is supported by the observation that procoat is synthesized exclusively by polysomes that are not membrane bound. These results are interpreted in terms of the "membrane-triggered folding" hypothesis of membrane protein assembly.

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Citations

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E Crooke, W Wickner
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K A Sevarino, R O Poyton
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T Date, W Wickner
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M Müller, G Blobel
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D G PuttermanL A Day
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C MarchalM Hofnung
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T DateW Wickner
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T DateW T Wickner
Mar 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Russel, P Model
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Oct 25, 1985·Science·W T Wickner, H F Lodish

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