Soluble tumor necrosis factor receptors are present in human vitreous and shed by retinal pigment epithelial cells

Experimental Eye Research
B D SippyD R Hinton

Abstract

Tumor necrosis factor-alpha (TNF) has been implicated in the pathogenesis of several retinal diseases. Soluble forms of the TNF receptors, p55 (55 kDa) and p75 (75 kDa), have recently been identified in biological fluids and may regulate TNF activity. The potential biological significance of these receptors for the human retina was examined by determining their presence in human vitreous and their release from eye cup explants in which the retina has been removed leaving an intact retinal pigment epithelium (HRPE). Normal human vitreous and conditioned medium from eye-cup HRPE explants demonstrated the presence of soluble p55 and p75. Soluble p55 was significantly more abundant than p75 in all vitreous samples (P < 0.03). Conditioned medium from eye-cup HRPE explants contained significantly more soluble p55 than p75 (P < 0.00002). Enzyme-linked immunosorbent assay showed the presence of soluble p55, and not p75, in conditioned medium from primary cultured HRPE cells. Activation of the protein kinase C pathway in these cells with the phorbol ester PMA significantly increased the release of soluble p55 (P < or = 0.001); whereas, pharmacological inhibition of protein kinase C with calphostin-C significantly decreased the shedding ...Continue Reading

Citations

Feb 1, 2017·Expert Review of Clinical Immunology·Kara C LaMattina, Debra A Goldstein
Aug 29, 2001·Journal of Cataract and Refractive Surgery·X H ZhangJ Q Yuan
Apr 18, 2009·Seminars in Ophthalmology·Yannek I Leiderman, Joan W Miller
Aug 26, 1998·Biochemical and Biophysical Research Communications·P M HeD R Hinton
Nov 9, 2000·Progress in Retinal and Eye Research·G M HoltkampA F de Vos

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