PMID: 8605587Apr 15, 1996Paper

Soluble tumor necrosis factor receptors inhibit phorbol myristate acetate and cytokine-induced HIV-1 expression chronically infected U1 cells

Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association
E V GranowitzP R Skolnik

Abstract

Recombinant human tumor necrosis factor (TNF) binding protein-1 (r-h TBP-1) and recombinant human soluble dimeric TNF receptor (rhu TNFR:Fc) were used to determine the relative contributions of TNF to phorbol myristate acetate (PMA) and cytokine-induced human immunodeficiency virus type 1 (HIV-1) replication in chronically infected cell lines. Treatment of HIV-1-infected promonocytic U1 cells with r-h-TBP-1 or rhu TNFR:Fc reduced PMA-induced HIV-1 p24 antigen production in a concentration-dependent manner, with a maximal inhibition of approximately 90%. Maximal inhibition of p24 antigen production in T-lymphocytic ACH-2 cells was 47% with r-hTBP-1 and 42% with rhu TNFR:Fc. r-hTBP-1 and rhu TNFR:Fc also decreased p24 antigen synthesized by U1 cells in response to other stimuli, including phytohemagglutinin (PHA)-induced supernatant, granulocyte-macrophage colony-stimulating factor, interleukin-6, and TNF. Addition of r-hTBP-1 to U1 cells during the last 4 h of a 24 h incubation with PMA still inhibited p24 antigen production by 15%. U1 cells stimulated with 10(-7) M PMA released approximately 1 ng/ml endogenous TBP-1 with an initial peak observed at 1 h and a second peak at 24 h after PMA stimulation. r-hTBP-1 also partially rev...Continue Reading

References

Feb 1, 1992·The Journal of Experimental Medicine·D AderkaD Wallach
Nov 1, 1991·Cytokine·W L FarrarK A Clouse
Apr 1, 1991·The Journal of Infectious Diseases·B MøllerC M Petersen
Jun 1, 1993·Seminars in Immunology·G Poli, A S Fauci
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S MakonkawkeyoonG Kaplan
Sep 1, 1993·Clinical and Experimental Immunology·A KalinkovichZ Bentwich

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Citations

Mar 10, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·S GradsteinZ T Handzel
Jan 31, 2002·HIV Clinical Trials·UNKNOWN Forum for Collaborative HIV Research.
Apr 8, 1999·Journal of Applied Physiology·R RoubenoffS Gorbach

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