Soluble yerba mate (Ilex Paraguariensis) extract enhances in vitro osteoblastic differentiation of bone marrow-derived mesenchymal stromal cells

Journal of Ethnopharmacology
Victor Gustavo Balera BritoSandra Helena Penha Oliveira

Abstract

Yerba mate (Ilex paraguariensis) consumption has been associated with beneficial effects on bone health. The purpose of this study was to evaluate the mechanism by which soluble yerba mate (SYM) stimulates osteoblast differentiation of bone marrow-derived mesenchymal stromal cells (BM-MSCs). BM-MSCs from male Wistar rats were induced towards osteoblastic differentiation with different concentrations of SYM (10, 20, and 50 μg/mL). Osteoblastic differentiation was evaluated by measuring proliferation rates, alkaline phosphatase activity, MMP-2 activity, mineralization, and gene expression of Runx2, Osterix, β-catenin (Catnb), collagen type I (Col1a1), osteopontin (Opn), osteocalcin (Ocn), bone sialoprotein (Bsp), bone morphogenetic protein-2 (Bmp2), osteoprotegerin (Opg), and Rankl. We also analyzed cytokine production and MAP kinase pathways. SYM (10 μg/mL) did not show a cytotoxic effect and induced a slight increase in ALP activity; however, a great increase in mineralization was observed. SYM was also able to reduce TNF-α and IL-10 production; increase the expression of transcription factors Runx2, Osterix, and Catnb; and increase matrix proteins Opn, Bsp, Ocn, and Bmp2. We also observed a decrease in intracellular signaling ...Continue Reading

References

Nov 1, 1988·Cell and Tissue Research·C ManiatopoulosA H Melcher
Feb 1, 1985·Toxicology Letters·E Borenfreund, J A Puerner
Feb 8, 2000·The Journal of Biological Chemistry·G XiaoR T Franceschi
Feb 19, 2000·Biochemical and Biophysical Research Communications·J LouP R Manske
Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A MizutaniN Tsukagoshi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 6, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Chaoxiang ShuiSundeep Khosla
May 17, 2005·Fitoterapia·Nicole Lunceford, Alejandro Gugliucci
Aug 31, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Aaron Schindeler, David G Little
Jun 21, 2007·Journal of Cellular Physiology·Lee A KabackHicham Drissi
Jul 20, 2007·Arthritis Research & Therapy·Brendan F Boyce, Lianping Xing
Sep 14, 2007·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Deborah H Markowicz BastosMarcos N Eberlin
Mar 11, 2008·Molecular and Cellular Biochemistry·Masayoshi YamaguchiTaeko Nakagawa
Nov 20, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tetsuya MatsuguchiTomokazu Ohnishi
Aug 4, 2009·Journal of Cellular Biochemistry·Katie L PricolaRocky S Tuan
Sep 29, 2009·Archives of Pharmacal Research·Guang-Hua XuIck-Dong Yoo
Jul 6, 2010·Journal of Ethnopharmacology·N BracescoA Gugliucci
Jul 14, 2010·Journal of Cellular Biochemistry·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira
Aug 7, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira
Sep 11, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Hyung Min JeongHye Gwang Jeong
Apr 23, 2011·Journal of Agricultural and Food Chemistry·Kleber A S BertéRosemary Hoffmann-Ribani
May 2, 2012·Chinese Medicine·Ava Jiangyang GuoKarl Wah-Keung Tsim
Jun 26, 2013·Food Chemistry·Demétrius Paiva ArçariMarcelo Lima Ribeiro
Sep 17, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Quan YuanBeate Lanske
Jan 21, 2014·BMC Complementary and Alternative Medicine·Yoshiki MukudaiSatoru Shintani
Jun 7, 2014·Journal of Inflammation·Helen VesterClaudine Seeliger
Mar 18, 2015·Journal of Cellular Biochemistry·Melissa B RogersNadia N Shaikh
May 8, 2016·International Immunopharmacology·Ana Beatriz Gobbo LuzEduardo Monguilhott Dalmarco
Jan 18, 2017·Experimental Gerontology·Ariana Aparecida Ferreira PereiraAna Cláudia de Melo Stevanato Nakamune
Feb 1, 2017·Molecular Medicine Reports·Li HuangJinmin Zhao
Jul 26, 2017·Experimental Gerontology·Camila Scacco PereiraAna Cláudia de Melo Stevanato Nakamune
Oct 17, 2017·Clinical Oral Investigations·Matheus da Silva BrasilinoAntonio Hernandes Chaves-Neto
Nov 9, 2017·Oncotarget·Xiangbing MaoJun He

❮ Previous
Next ❯

Citations

Jun 3, 2021·Antioxidants·Chanchao LorthongpanichSurapol Issaragrisil
Jul 17, 2021·Tissue Engineering. Part a·Nolutho MkhumbeniKeolebogile Shirley Caroline Motaung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Related Papers

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Esther LauLidan You
Journal of Biomedical Materials Research. Part a
Daniel G CostaAdalberto L Rosa
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Mei-Jie ShenXi Ding
© 2021 Meta ULC. All rights reserved