PMID: 9177057Jan 1, 1997Paper

Solute accumulation and decreased photosynthesis in leaves of potato plants expressing yeast-derived invertase either in the apoplast, vacuole or cytosol

Planta
D BüssisH W Heldt

Abstract

Potato (Solanum tuberosum cv. Désirée) plants expressing yeast invertase directed either to the apoplast, vacuole or cytosol were biochemically and physiologically characterised. All lines of transgenic plants showed similarities to plants growing under water stress. Transformants were retarded in growth, and accumulated hexoses and amino acids, especially proline, to levels up to 40-fold higher than those of the wild types. In all transformants rates of CO2 assimilation and leaf conductance were reduced. From the unchanged intercellular partial pressure of CO2 and apoplastic cis-abscisic acid (ABA) content of transformed leaves it was concluded that the reduced rate of CO2 assimilation was not caused by a limitation in the availability of CO2 for the ribulose-1,5-bisphosphate carboxylase-oxygenase (Rubisco). In the transformants the amount of Rubisco protein was not reduced, but both activation state and carboxylation efficiency of photosynthesis were lowered. In vacuolar and cytosolic transformants this inhibition of Rubisco might be caused by a changed ratio of organic bound and inorganic phosphate, as indicated by a doubling of phosphorylated intermediates. But in apoplastic transformants the pattern of phosphorylated inter...Continue Reading

Citations

Mar 6, 1998·Nature Biotechnology·M X CaddickA B Tomsett
Aug 1, 1997·Nature Biotechnology·U SonnewaldL Willmitzer
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Wolfgang KochMechthild Tegeder
Sep 29, 2011·Plant Physiology·Takayuki TohgeAlisdair R Fernie
Jun 13, 2002·Physiologia Plantarum·Fausto ManesMarcello Vitale
Mar 2, 2011·Molecular Plant Pathology·Johannes SiemensJutta Ludwig-Müller
Apr 17, 2016·Journal of Experimental Botany·Domenica FarciDario Piano
Mar 18, 2015·Bioscience, Biotechnology, and Biochemistry·Masahiro Tamoi, Shigeru Shigeoka
Mar 26, 2002·Journal of Experimental Botany·Graham NoctorChristine H Foyer
Jul 31, 2019·The Plant Journal : for Cell and Molecular Biology·Lisa FürtauerThomas Nägele

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