Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo.

Proceedings of the National Academy of Sciences of the United States of America
Aya TakesonoTetsuhiro Kudoh

Abstract

The yolk syncytial layer (YSL) in the zebrafish embryo is a multinucleated syncytium essential for embryo development, but the molecular mechanisms underlying YSL formation remain largely unknown. Here we show that zebrafish solute carrier family 3 member 2 (Slc3a2) is expressed specifically in the YSL and that slc3a2 knockdown causes severe YSL defects including clustering of the yolk syncytial nuclei and enhanced cell fusion, accompanied by disruption of microtubule networks. Expression of a constitutively active RhoA mimics the YSL phenotypes caused by slc3a2 knockdown, whereas attenuation of RhoA or ROCK activity rescues the slc3a2-knockdown phenotypes. Furthermore, slc3a2 knockdown significantly reduces tyrosine phosphorylation of c-Src, and overexpression of a constitutively active Src restores the slc3a2-knockdown phenotypes. Our data demonstrate a signaling pathway regulating YSL formation in which Slc3a2 inhibits the RhoA/ROCK pathway via phosphorylation of c-Src to modulate YSL microtubule dynamics. This work illuminates processes at a very early stage of zebrafish embryogenesis and more generally informs the mechanism of cell dynamics during syncytium formation.

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Citations

Apr 16, 2013·Analytical Chemistry·Jessica C MansfieldJulian Moger
Jan 9, 2013·The Journal of Experimental Medicine·Etienne BoulterChloé C Féral
Jul 5, 2017·Zygote : the Biology of Gametes and Early Embryos·Ekaterina KondakovaVladimir Efremov
Oct 15, 2013·Journal of Morphology·Ekaterina Alexandrovna A Kondakova, Vladimir Ivanovich I Efremov
Dec 5, 2018·Development·Zhonghui FeiAshley E E Bruce
Jun 6, 2017·Nature Communications·Germán ReigMiguel L Concha
Nov 29, 2017·Development·James A GagnonAlexander F Schier
Aug 16, 2017·Scientific Reports·Hongyuan ShenVladimir Korzh
Dec 12, 2020·Cells·Marie-José BoueidMarcel Tawk
Nov 19, 2020·Developmental Cell·Benoit G GodardCarl-Philipp Heisenberg

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