Solute effects on spin labels at an aqueous-exposed site in the flap region of HIV-1 protease

The Journal of Physical Chemistry. B
Luis GalianoGail E Fanucci

Abstract

The effects of solutes on spin-label mobility and protein conformation have been investigated with X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) spectroscopy for spin labels attached to an aqueous-exposed site in the beta-hairpin flap region of HIV-1 protease. Specifically, we examined the effects of glycerol, sucrose, PEG3000, and Ficoll400 for four commonly used nitroxide spin labels and found that the largest perturbations to the EPR line shapes occur for solutions containing PEG3000 and glycerol. From comparisons of the spectral line shapes and distance distribution profiles of spin-labeled HIV-1 protease with and without inhibitor, it was concluded that solutes such as glycerol and PEG3000 alter the line shapes of the spin label in the beta-hairpin flaps of HIV-1 PR by modulation of spin-label mobility through changes in preferential interactions with the solutes. It is noteworthy that the high osmolality of the 40% glycerol solution did not alter the conformation of the flaps as determined from pulsed EPR distance measurements.

References

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P AshornB Moss
Nov 19, 1985·Biochemistry·T Arakawa, S N Timasheff
Sep 5, 1981·Journal of Molecular Biology·G C Na, S N Timasheff
Jul 15, 1996·Structure·W L HubbellM A Lietzow
Aug 7, 1999·The Journal of Biological Chemistry·J M LouisN T Nashed
Jan 29, 2000·Journal of Magnetic Resonance·M PannierH W Spiess
Mar 10, 2000·Biochimica Et Biophysica Acta·A Wlodawer, A Gustchina
Apr 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·V A ParsegianD C Rau
Aug 31, 2000·Nature Structural Biology·W L HubbellC Altenbach
Feb 17, 2001·Current Opinion in Structural Biology·R J Ellis
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Jun 19, 2002·Trends in Biochemical Sciences·Linda Columbus, Wayne L Hubbell
Jul 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Serge N Timasheff
Jun 5, 2003·Biochemistry·Peter Z QinWayne L Hubbell
Jun 26, 2003·Protein Science : a Publication of the Protein Society·Etsuko KatohRieko Ishima
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Jan 15, 2005·Journal of Magnetic Resonance·Yun-Wei ChiangJack H Freed
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Viktor HornakCarlos Simmerling
Jan 31, 2006·Biophysical Journal·Miyeon KimDavid S Cafiso
Mar 15, 2006·Biochemical and Biophysical Research Communications·Peter Z QinArisa Oki
Apr 25, 2006·Current Biology : CB·Allen P Minton
Jul 11, 2006·Journal of Cell Science·Allen P Minton
Sep 5, 2006·Current Opinion in Structural Biology·Gail E Fanucci, David S Cafiso
Dec 26, 2006·Biophysical Journal·Noga KozerGideon Schreiber
May 29, 2007·Biophysical Journal·Qi CaiPeter Z Qin
Aug 21, 2007·Journal of the American Chemical Society·Luis GalianoGail E Fanucci
Oct 2, 2007·Biophysical Chemistry·Tsutomu ArakawaSerge N Timasheff
Feb 9, 2008·Chembiochem : a European Journal of Chemical Biology·Peter B CrowleyJimmy Muldoon
Jun 25, 2008·Annual Review of Biophysics·Huan-Xiang ZhouAllen P Minton

❮ Previous
Next ❯

Citations

Jun 5, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Gunnar Jeschke
Sep 20, 2011·Chemical Reviews·Jeeyeon Lee, Nina M Goodey
Mar 13, 2012·Annual Review of Physical Chemistry·Gunnar Jeschke
Jan 26, 2013·Journal of the American Chemical Society·John M FranckSongi Han
Nov 17, 2010·Protein Science : a Publication of the Protein Society·Natasha L PirmanGail E Fanucci
Feb 13, 2015·Bioinformatics·Elihu C Ihms, Mark P Foster
Jul 28, 2016·Journal of the American Chemical Society·Shu-Hao LiouDavid B Goodin
Oct 23, 2015·Physical Chemistry Chemical Physics : PCCP·Zhanglong LiuGail E Fanucci
Mar 1, 2018·European Biophysics Journal : EBJ·Antonia FloresYong Ba
Apr 20, 2014·The Journal of Biological Chemistry·Xi HuangGail E Fanucci
Feb 11, 2014·Current Protocols in Protein Science·Ian Mitchelle S de VeraGail E Fanucci
Apr 20, 2010·The Journal of Physical Chemistry. B·Zhongyu YangSunil Saxena
Apr 10, 2013·Biochemistry·Ian Mitchelle S de VeraGail E Fanucci

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.