Solution and crystal structures of a sperm whale myoglobin triple mutant that mimics the sulfide-binding hemoglobin from Lucina pectinata.

The Journal of Biological Chemistry
B D NguyenJ B Wittenberg

Abstract

The bivalve mollusc Lucina pectinata harbors sulfide-oxidizing chemoautotrophic bacteria and expresses a monomeric hemoglobin I, HbI, with normal O2, but extraordinarily high sulfide affinity. The crystal structure of aquomet Lucina HbI has revealed an active site with three residues not commonly found in vertebrate globins: Phe(B10), Gln(E7), and Phe(E11) (Rizzi, M., Wittenberg, J. B., Coda, A., Fasano, M., Ascenzi, P., and Bolognesi, M. (1994) J. Mol. Biol. 244, 86-89). Engineering these three residues into sperm whale myoglobin results in a triple mutant with approximately 700-fold higher sulfide affinity than for wild-type. The single crystal x-ray structure of the aquomet derivative of the myoglobin triple mutant and the solution 1H NMR active site structures of the cyanomet derivatives of both the myoglobin mutant and Lucina HbI have been determined to examine further the structural origin of their unusually high sulfide affinities. The major differences in the distal pocket is that in the aquomet form the carbonyl of Gln64(E7) serves as a H-bond acceptor, whereas in the cyanomet form the amido group acts as H-bond donor to the bound ligand. Phe68(E11) is rotated approximately 90 degrees about chi2 and located approximate...Continue Reading

References

Jan 1, 1990·Annual Review of Biophysics and Biophysical Chemistry·J B Wittenberg, B A Wittenberg
Jan 1, 1990·Proteins·G N PhillipsS G Sligar
Nov 5, 1989·Journal of Molecular Biology·G Arents, W E Love
Feb 5, 1989·Journal of Molecular Biology·M BolognesiM Brunori
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B A Springer, S G Sligar
Jul 5, 1987·Journal of Molecular Biology·D BashfordA M Lesk
Nov 5, 1986·Journal of Molecular Biology·J KuriyanG A Petsko
Jan 1, 1981·Journal of Molecular Evolution·A E Romero-HerreraH Mizukami
Jan 27, 1995·Journal of Molecular Biology·M L QuillinH Li
Sep 1, 1993·Comparative Biochemistry and Physiology. Comparative Physiology·V CherepanovaR Elsner
Nov 5, 1993·Journal of Molecular Biology·M L QuillinG N Phillips
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S N VinogradovC N Trotman
Apr 26, 1996·Journal of Molecular Biology·M RizziM Bolognesi
Jan 31, 1997·The Journal of Biological Chemistry·A K RashidL Moens
Jan 23, 1987·Science·A T BrüngerM Karplus

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Apr 17, 2009·Biochemistry·Murugaeson R KumarPatrick J Farmer
Jul 10, 2009·Journal of the American Chemical Society·Luyuan ZhangDongping Zhong
Nov 6, 2010·Antioxidants & Redox Signaling·Ruth PietriJuan López-Garriga
Jan 11, 2012·Antioxidants & Redox Signaling·Victor VitvitskyRuma Banerjee
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Jul 20, 2012·Biochemical and Biophysical Research Communications·Brenda J Ramos-Santana, Juan López-Garriga
Feb 9, 2005·Biochimica Et Biophysica Acta·Ruth PietriJuan López Garriga
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Jun 20, 2006·Biophysical Journal·S Fernandez-AlbertiJ Lopez-Garriga
Dec 30, 2014·Inorganic Chemistry·Silvina A BiezaSara E Bari
Dec 18, 2001·The Journal of Biological Chemistry·Federica DraghiJohn S Olson
Apr 12, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Vasyl BondarenkoGerd N La Mar
Jan 14, 2010·Prikladnaia biokhimiia i mikrobiologiia·O V Kosmachevskaia, A F Topunov
Mar 29, 2001·Physiological Reviews·R E Weber, S N Vinogradov
Mar 24, 2021·Inorganic Chemistry·Tapiwa Chiura, Piotr J Mak
Jan 28, 2010·Biochemistry·Francesco P NicolettiAlberto Boffi
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee

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