Abstract
Three different conformations of proline rings in a protein in solution, Up, Down and Twist, have been distinguished, and stereospecific assignments of the pyrrolidine beta-, gamma- and delta-hydrogens have been made on the basis of 1H-1H vicinal coupling constant patterns and intraresidue NOEs. For all three conformations, interhydrogen distances in the pairs alpha-beta 3, beta 3-gamma 3, beta 2-gamma 2, gamma 2-delta 2, and gamma 3-delta 3 (2.3 A) are shorter than those in the pairs alpha-beta 2, beta 2-gamma 3, beta 3-gamma 2, gamma 2-delta 3, and gamma 3-delta 2 (2.7-3.0 A), resulting in stronger NOESY cross peaks. For the Up conformation, the beta 3-gamma 2 and gamma 2-delta 3 spin-spin coupling constants are small (< 3 Hz), and weak cross peaks are obtained in a short-mixing-time (10 ms) TOCSY spectrum; all other vicinal coupling constants are in the range 5-12 Hz, and result in medium to strong TOCSY cross peaks. For the Down form, the alpha-beta 2, beta 2-gamma 3, and gamma 3-delta 2 vicinal coupling constants are small, leading to weak TOCSY cross peaks; all other couplings again are in the range 5-12 Hz, and result in medium to strong TOCSY cross peaks. In the case of a Twist conformation, dynamically averaged couplin...Continue Reading
References
Oct 5, 1992·Journal of Molecular Biology·K D BerndtK Wüthrich
Dec 5, 1992·Journal of Molecular Biology·E J Milner-WhiteP H Maccallum
Mar 5, 1991·Biochemistry·G M CloreA M Gronenborn
Feb 20, 1990·Biochemistry·G M CloreA M Gronenborn
Oct 29, 1990·FEBS Letters·R KrishnamoorthiM Richardson
Jan 1, 1990·Biopolymers·K A Thomasson, J Applequist
Aug 5, 1987·Journal of Molecular Biology·G WagnerK Wüthrich
Dec 5, 1987·Journal of Molecular Biology·A WlodawerC Woodward
Jan 1, 1989·Methods in Enzymology·V J Basus
Jan 1, 1989·Annual Review of Biochemistry·A Bax
Sep 19, 1989·Biochemistry·D NeriK Wüthrich
Nov 20, 1988·Journal of Molecular Biology·P L WeberD Hare
May 17, 1988·Biochemistry·K V CharyH T Miles
Sep 1, 1986·FEBS Letters·R V HosurH T Miles
Jul 16, 1980·Biochemical and Biophysical Research Communications·A KumarK Wüthrich
May 1, 1995·Journal of Magnetic Resonance. Series B·M CaiR Krishnamoorthi
Apr 18, 1995·Biochemistry·M CaiR Krishnamoorthi
Mar 1, 1993·FEBS Letters·G OstlerJ Feeney
Citations
Feb 13, 2003·Journal of Molecular Biology·Matthew H J CordesRobert T Sauer
Jan 21, 2011·Biochemistry·Hanke van der WelChristopher M West
May 8, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·G MoynaA I Scott
Nov 18, 2000·The Journal of Peptide Research : Official Journal of the American Peptide Society·M J PotrzebowskiM W Wieczorek
Apr 1, 2010·Physical Chemistry Chemical Physics : PCCP·Hui ZhuBurkhard Schmidt
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Lucia BanciMaria Silvia Viezzoli
Aug 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·H Jane DysonHarold A Scheraga
Apr 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel J FelitskyPeter E Wright
Jun 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sina ReckelVolker Dötsch
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Riechmann, G Winter
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·A ShimadaS Yokoyama
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·D G DonneH J Dyson
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Srinivasan, G D Rose
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H LaityP E Wright
Apr 20, 1999·The Journal of Experimental Medicine·M LéonettiA Ménez
Nov 26, 2009·Glycobiology·Leticia F HallackAdriane R Todeschini
Jan 18, 2006·Biopolymers·A K Thakur, R Kishore
Jul 5, 2006·The FEBS Journal·Zakhar O ShenkarevAlexander S Arseniev
Jul 20, 2016·Journal of the American Chemical Society·Andreas SeegererRuth M Gschwind
Jan 21, 2017·Chemistry : a European Journal·Maic FredersdorfChristina Marie Thiele
Feb 24, 2015·Organic & Biomolecular Chemistry·Vladimir KubyshkinAnne S Ulrich
Oct 29, 2014·Chemistry : a European Journal·Yujiro HayashiDieter Seebach
Dec 14, 2016·Organic & Biomolecular Chemistry·Vladimir Kubyshkin, Nediljko Budisa
Jul 20, 2002·Photochemistry and Photobiology·Adam WrightMichael J Davies
Aug 4, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Aliona DobrovaVladimir B Arion
Aug 3, 2017·Organic & Biomolecular Chemistry·Vladimir Kubyshkin, Nediljko Budisa
Feb 22, 2000·FEBS Letters·T A BalashovaA S Arseniev
Jan 22, 2009·Chembiochem : a European Journal of Chemical Biology·Markus B SchmidRuth M Gschwind
Sep 10, 2009·Chemistry : a European Journal·Neil W OwensFrank Schweizer
Oct 22, 2002·Biopolymers·Brandon A WustmanJohn Spencer Evans
Mar 20, 2003·Journal of Biomolecular Structure & Dynamics·Arnd B E BrauerRobin J Leatherbarrow
Jun 25, 2004·Journal of Biomolecular Structure & Dynamics·T KaiT Tomiyama
Jul 30, 2002·Journal of Biomolecular Structure & Dynamics·Arnd B E BrauerRobin J Leatherbarrow
Aug 9, 2020·Beilstein Journal of Organic Chemistry·Vladimir Kubyshkin
Dec 14, 2017·Beilstein Journal of Organic Chemistry·Vladimir Kubyshkin, Nediljko Budisa
Feb 6, 1996·Biochemistry·J LiuR Krishnamoorthi
Mar 13, 2001·Journal of Molecular Biology·A B BrauerR J Leatherbarrow
Aug 15, 2018·Journal of the American Chemical Society·Carla RiglingHelma Wennemers
Sep 25, 2014·Journal of the American Chemical Society·Chamini V KarunaratneCarol M Taylor