Solution NMR structure of the C-terminal domain of the human protein DEK

Protein Science : a Publication of the Protein Society
Matthew DevanyHiroshi Matsuo

Abstract

The chromatin-associated protein DEK was first identified as a fusion protein in patients with a subtype of acute myelogenous leukemia. It has since become associated with diverse human ailments ranging from cancers to autoimmune diseases. Despite much research effort, the biochemical basis for these clinical connections has yet to be explained. We have identified a structural domain in the C-terminal region of DEK [DEK(309-375)]. DEK(309-375) implies clinical importance because it can reverse the characteristic abnormal DNA-mutagen sensitivity in fibroblasts from ataxia-telangiectasia (A-T) patients. We determined the solution structure of DEK(309-375) by nuclear magnetic resonance spectroscopy, and found it to be structurally homologous to the E2F/DP transcription factor family. On the basis of this homology, we tested whether DEK(309-375) could bind DNA and identified the DNA-interacting surface. DEK presents a hydrophobic surface on the side opposite the DNA-interacting surface. The structure of the C-terminal region of DEK provides insights into the protein function of DEK.

References

Oct 1, 1992·Baillière's Clinical Haematology·M von LindernG Grosveld
May 1, 1991·Archives of Physical Medicine and Rehabilitation·A K LibbyB J Dudgeon
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D M MarkovitzG J Nabel
Mar 15, 1989·Biochemical and Biophysical Research Communications·S W FesikD Weigl
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Apr 8, 1998·Current Opinion in Genetics & Development·K Helin
Jan 22, 2000·Arthritis and Rheumatism·X DongW H Reeves
Feb 19, 2000·Current Opinion in Structural Biology·K S Gajiwala, S K Burley
Sep 12, 2000·Bioinformatics·L Holm, J Park
Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·C GrottkeH Lage
May 16, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·W H BiggsK C Arden
Sep 26, 2002·Developmental Biology·Peter Carlsson, Margit Mahlapuu
Jun 26, 2003·Arthritis Research & Therapy·Barbara S AdamsDavid M Markovitz
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

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Citations

Aug 15, 2009·Cancer Research·Michael S KhodadoustMaría S Soengas
May 27, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Erica Riveiro-Falkenbach, María S Soengas
Feb 5, 2005·Journal of Biomolecular NMR·Matthew Devany, Hiroshi Matsuo
Aug 23, 2011·International Journal for Parasitology·Pamela CribbEsteban Serra
Nov 28, 2008·The American Journal of Pathology·Trisha M Wise-DraperSusanne I Wells
Jan 30, 2008·Protein Science : a Publication of the Protein Society·Matthew DevanyHiroshi Matsuo
Dec 21, 2012·Cell Cycle·Lisa M Privette VinnedgeSusanne I Wells
Mar 21, 2017·Scientific Reports·Eric A SmithSusanne I Wells
Sep 24, 2005·Genes, Chromosomes & Cancer·Marija OrlicBrenda L Gallie
May 21, 2019·The Journal of Clinical Investigation·Maegan L CapitanoHal E Broxmeyer
Apr 8, 2020·Molecular and Cellular Biology·Bruce A KnutsonAula M Fakhouri
Aug 3, 2017·Molecular Medicine Reports·Yang XuLiangan Chen

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