Abstract
In response to illumination by ultraviolet-A (UV-A) light, proteins in solid form are now known to display a visible blue fluorescence, ostensibly on account of excitation transitions of loosely-held electrons within peptide bond orbitals engaged in hydrogen bonding. Because the CO and NH atom groups in peptide bonds are generally engaged in extensive hydrogen bonding in globular proteins even in aqueous solution, one could argue that proteins in solution must also display this novel blue fluorescence. Here, using high concentrations to enhance detectability, two globular proteins, gamma-crystallin, and lysozyme, are shown to fluoresce visibly, exhibiting: (a) two excitation maxima, at approximately 315 nm and approximately 385 nm, (b) maximal emission at 425 nm in 100 mg/ml lysozyme and 465 nm in 100 mg/ml gamma-crystallin, (c) a time-resolved emission decay that is best fitted by a sum of three exponentials with lifetimes of 3.14, 0.46, and 9.08 ns, respectively, and comparable relative amplitudes of around 30-40 percent each, and (d) a weak CD spectrum displaying a positive band at approximately 385 nm and a negative band at approximately 465 nm. While the wavelength of maximal emission ((em)lambda(max)) in lysozyme is the s...Continue Reading
References
May 1, 1986·Die Naturwissenschaften·W LohmannK G Bensch
Oct 10, 1973·Experimental Eye Research·R B KurzelB S Yamanashi
Jul 1, 1972·Experimental Eye Research·K Satoh
Citations
Feb 14, 2012·Biochemistry·Pius PadayattiDavid Salom
Oct 21, 2011·ACS Nano·Kamalesh ChaudhariThalappil Pradeep
Feb 20, 2013·The Analyst·Fiona T S ChanClemens F Kaminski
Apr 13, 2010·Medical Hypotheses·Manni Luthra-Guptasarma, Purnananda Guptasarma
Oct 27, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Amir HandelmanGil Rosenman
Jan 7, 2016·PLoS Biology·Liangzhong LimJianxing Song
May 26, 2017·PloS One·Chamani NiyangodaMartin Muschol
Jan 31, 2018·Faraday Discussions·Shaina DhamijaArijit K De
Jan 24, 2018·Faraday Discussions·I MandalR Venkatramani
Jan 18, 2020·Journal of Biomolecular Structure & Dynamics·Evgeni L TerpugovOlga V Degtyareva
Feb 15, 2020·Angewandte Chemie·Songyuan TaoBai Yang
Oct 4, 2017·Chemical Science·Saumya PrasadRajaram Swaminathan
Apr 23, 2017·Scientific Reports·Yimei LuJianxing Song
Mar 2, 2021·The Analyst·Catarina S H JesusCarlos Serpa
Mar 7, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yunqian MaChuanjian Zhou