Solution structure and dynamics of Crh, the Bacillus subtilis catabolite repression HPr

Journal of Molecular Biology
A FavierDominique Marion

Abstract

The solution structure and dynamics of the Bacillus subtilis HPr-like protein, Crh, have been investigated using NMR spectroscopy. Crh exhibits high sequence identity (45 %) to the histidine-containing protein (HPr), a phospho-carrier protein of the phosphoenolpyruvate (PEP):carbohydrate phosphotransferase system, but contains no catalytic His15, the site of PEP-dependent phosphorylation in HPr. Crh also forms a mixture of monomers and dimers in solution whereas HPr is known to be monomeric. Complete backbone and side-chain assignments were obtained for the monomeric form, and 60 % of the dimer backbone resonances; allowing the identification of the Crh dimer interface from chemical-shift mapping. The conformation of Crh was determined to a precision of 0.46(+/-0.06) A for the backbone atoms, and 1.01(+/-0.08) A for the heavy atoms. The monomer structure is similar to that of known HPr 2.67(+/-0.22) A (C(alpha) rmsd), but has a few notable differences, including a change in the orientation of one of the helices (B), and a two-residue shift in beta-sheet pairing of the N-terminal strand with the beta4 strand. This shift results in a shortening of the surface loop present in HPr and consequently provides a flatter surface in the ...Continue Reading

References

Oct 11, 1992·Protein Science : a Publication of the Protein Society·M WittekindR E Klevit
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J Deutscher, M H Saier
Sep 1, 1993·Microbiological Reviews·P W PostmaG R Jacobson
Jan 19, 1996·The Journal of Biological Chemistry·I Ekiel, M Abrahamson
Dec 1, 1995·Protein Science : a Publication of the Protein Society·M J BennettD Eisenberg
Dec 1, 1995·Protein Science : a Publication of the Protein Society·K PullenR E Klevit
Feb 1, 1996·Protein Science : a Publication of the Protein Society·E G Hutchinson, J M Thornton
Mar 1, 1996·Protein Science : a Publication of the Protein Society·N A Van NulandG T Robillard
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Mar 15, 1997·Structure·M BergdollP Dumas
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·A GalinierI Martin-Verstraete
Aug 15, 1997·Journal of Molecular Biology·I EkielK Gehring
Oct 23, 1997·Protein Science : a Publication of the Protein Society·B E JonesR E Klevit
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·A GalinierJ Haiech
May 7, 1998·Molecular Microbiology·J ReizerW Hillen
Nov 13, 1999·Journal of Bacteriology·E Presecan-SiedelI Martin-Verstraete
May 4, 2000·Protein Science : a Publication of the Protein Society·H R KalbitzerK Akasaka
Jul 13, 2000·Protein Science : a Publication of the Protein Society·M L TillettT J Norwood
Oct 31, 2000·Journal of Molecular Biology·G F AudetteL T Delbaere

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Citations

Mar 6, 2008·Journal of Biomolecular NMR·Carole GardiennetAnja Böckmann
Jul 30, 2010·The Journal of Biological Chemistry·Roland MontserretFrançois Penin
Jul 23, 2008·The Journal of Biological Chemistry·Vlastimil JiraskoRalf Bartenschlager
May 7, 2004·Applied and Environmental Microbiology·M Graça SilveiraTjakko Abee
Dec 15, 2010·Journal of Virology·Anna AlbeckaJean Dubuisson
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jessica B Warner, Juke S Lolkema
Jan 1, 2010·Advances and Applications in Bioinformatics and Chemistry : AABC·Benjamin BardiauxThérèse E Malliavin
Dec 18, 2007·Magnetic Resonance in Chemistry : MRC·Anja Böckmann
May 18, 2006·The Journal of Biological Chemistry·Steeve BoulantJohn McLauchlan
Feb 27, 2003·The Journal of Biological Chemistry·Rosa FayosPatricia A Jennings
Jul 13, 2004·The Journal of Biological Chemistry·François PeninDarius Moradpour
Dec 1, 2005·The Journal of Biological Chemistry·Maria A SchumacherRichard G Brennan
Sep 16, 2004·Journal of the American Chemical Society·Nicolas GiraudLyndon Emsley
Jan 4, 2007·Journal of the American Chemical Society·Manuel EtzkornMarc Baldus
Dec 18, 2003·Journal of the American Chemical Society·Matthias ErnstBeat H Meier
Feb 21, 2008·Journal of the American Chemical Society·Antoine LoquetAnja Böckmann
Nov 13, 2004·Journal of the American Chemical Society·Manuel EtzkornMarc Baldus

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