Solution structure and structural dynamics of envelope protein domain III of mosquito- and tick-borne flaviviruses

Biochemistry
Shaoning YuJ Ching Lee

Abstract

The mosquito-borne West Nile (WNV) and dengue 2 (DEN2V) viruses and tick-borne Langat (LGTV) and Omsk hemorrhagic fever (OHFV) viruses are arthropod-borne flaviviruses (family Flaviviridae, genus Flavivirus). These viruses are quite similar at both the nucleotide and amino acid level, yet they are very divergent in their biological properties and in the diseases they cause. The objective of this study was to examine the putative receptor-binding domains of the flaviviruses, the envelope (E) protein domain III (D3), which assume very similar structures either as part of the whole envelope protein or as individual entities, and to define the biophysical properties that distinguish among these viruses. Circular dichroism and Fourier transform infrared spectroscopy were employed to monitor the solution structure of these proteins. While the spectroscopic results found that the D3 from each of these viruses is composed of either beta-sheets or beta-turns, which is consistent with X-ray crystal data for tick-borne encephalitis and dengue viruses, these results reveal that recombinant D3s (rED3s) derived from tick-borne flaviviruses (LGT-rED3 and OHF-rED3) were similar to each other, while those from mosquito-borne flaviviruses (WN-rE...Continue Reading

References

Jun 9, 2004·Journal of Clinical Microbiology·David W C BeasleyAlan D T Barrett

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Citations

Mar 13, 2013·Protein Engineering, Design & Selection : PEDS·Nora ZidaneHugues Bedouelle
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Fang YuanGeorge F Gao
Jul 2, 2010·Journal of Virology·Soila Sukupolvi-PettyMichael S Diamond
Jun 29, 2010·PLoS Neglected Tropical Diseases·Joshua M CostinScott F Michael
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Sep 8, 2010·Biologicals : Journal of the International Association of Biological Standardization·Juliana Helena ChávezLuiz Tadeu Moraes Figueiredo
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May 4, 2005·Virology·Melissa D SánchezRobert W Doms
May 2, 2006·Virology·David E VolkDavid G Gorenstein
Aug 10, 2005·Protein Engineering, Design & Selection : PEDS·Elodie Monsellier, Hugues Bedouelle
Feb 27, 2020·Reviews in Medical Virology·Arianna Mahely Hurtado-MonzónRosa María Del Ángel

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