Solution structure of mouse hepatitis virus (MHV) nsp3a and determinants of the interaction with MHV nucleocapsid (N) protein

Journal of Virology
Sarah C Keane, David P Giedroc


Coronaviruses (CoVs) are positive-sense, single-stranded, enveloped RNA viruses that infect a variety of vertebrate hosts. The CoV nucleocapsid (N) protein contains two structurally independent RNA binding domains, designated the N-terminal domain (NTD) and the dimeric C-terminal domain (CTD), joined by a charged linker region rich in serine and arginine residues (SR-rich linker). An important goal in unraveling N function is to molecularly characterize N-protein interactions. Recent genetic evidence suggests that N interacts with nsp3a, a component of the viral replicase. Here we present the solution nuclear magnetic resonance (NMR) structure of mouse hepatitis virus (MHV) nsp3a and show, using isothermal titration calorimetry, that MHV N219, an N construct that extends into the SR-rich linker (residues 60 to 219), binds cognate nsp3a with high affinity (equilibrium association constant [K(a)], [1.4 ± 0.3] × 10(6) M(-1)). In contrast, neither N197, an N construct containing only the folded NTD (residues 60 to 197), nor the CTD dimer (residues 260 to 380) binds nsp3a with detectable affinity. This indicates that the key nsp3a binding determinants localize to the SR-rich linker, a finding consistent with those of reverse genetic...Continue Reading


Nov 1, 1979·Journal of Virology·S A Stohlman, M M Lai
Nov 1, 1988·Journal of Virology·S A StohlmanR J Deans
May 1, 1985·The Journal of General Virology·A SánchezA K Banerjee
Jun 1, 1987·Journal of Virology·S R ComptonJ J McGowan
Feb 1, 1981·The Journal of General Virology·S G SiddellV ter Meulen
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
May 8, 1998·Journal of Magnetic Resonance·M OttigerA Bax
Jul 22, 1998·Journal of Molecular Biology·J L MarkleyK Wüthrich
Dec 22, 1999·Journal of Molecular Biology·N BlomS Brunak
Oct 25, 2000·Protein Science : a Publication of the Protein Society·C A EarhartD H Ohlendorf
Feb 5, 2003·Journal of Magnetic Resonance·Charles D SchwietersG Marius Clore
Nov 13, 2003·Journal of the American Chemical Society·Eriks Kupce, Ray Freeman
Mar 24, 2004·Nature Reviews. Microbiology·Konrad StadlerRino Rappuoli
Mar 26, 2004·Journal of Magnetic Resonance·Homayoun Valafar, James H Prestegard
May 20, 2004·Journal of the American Chemical Society·Eriks Kupce, Ray Freeman
Aug 20, 2004·Methods in Molecular Biology·Peter Güntert
May 14, 2005·Journal of Virology·Barbara SchelleVolker Thiel
Jul 13, 2006·Journal of the American Chemical Society·Paul SchandaBernhard Brutscher
Aug 1, 2006·Advances in Virus Research·Paul S Masters
May 1, 2007·Journal of Magnetic Resonance·Ewen LescopBernhard Brutscher
Feb 22, 2008·Journal of Molecular Biology·Lichun LiDavid P Giedroc
Mar 19, 2008·Journal of the American Chemical Society·Ewen LescopBernhard Brutscher
Feb 10, 2009·Nature Reviews. Microbiology·Lanying DuShibo Jiang
Aug 27, 2010·Journal of Virology·Monique H VerheijeCornelis A M de Haan

❮ Previous
Next ❯


Jan 15, 2014·Antiviral Research·Chung-ke ChangTai-huang Huang
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Sep 10, 2013·Antiviral Research·Rolf Hilgenfeld, Malik Peiris
Mar 27, 2015·Journal of Virology·Kelley R Hurst-HessPaul S Masters
Jan 22, 2021·Biomolecular NMR Assignments·Nicola SalviMartin Blackledge

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.