Solution structure of polyglutamine tracts in GST-polyglutamine fusion proteins

FEBS Letters
Laura MasinoAnnalisa Pastore

Abstract

Aggregation of expanded polyglutamine (polyQ) seems to be the cause of various genetic neurodegenerative diseases. Relatively little is known as yet about the polyQ structure and the mechanism that induces aggregation. We have characterised the solution structure of polyQ in a proteic context using a model system based on glutathione S-transferase fusion proteins. A wide range of biophysical techniques was applied. For the first time, nuclear magnetic resonance was used to observe directly and selectively the conformation of polyQ in the pathological range. We demonstrate that, in solution, polyQs are in a random coil conformation. However, under destabilising conditions, their aggregation behaviour is determined by the polyQ length.

References

Nov 1, 1992·Journal of Biomolecular NMR·M PiottoV Sklenár
Jan 1, 1986·Methods in Enzymology·J T YangH M Martinez
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M F PerutzJ T Finch
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Jul 1, 1997·The Journal of Peptide Research : Official Journal of the American Peptide Society·E L AltschulerB Rupp
Jan 1, 1997·Methods in Enzymology·E E AbolaN O Manning
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y GeorgalisE E Wanker
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y W ChenM F Perutz
Mar 31, 1999·Trends in Biochemical Sciences·M F Perutz
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerE E Wanker
Aug 6, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B HollenbachE E Wanker
Jun 9, 2000·Annual Review of Neuroscience·H Y Zoghbi, H T Orr
Feb 13, 2001·Biochemical and Biophysical Research Communications·D J Gordon-SmithD Neuhaus
Mar 17, 2001·Nature Reviews. Neuroscience·J F Gusella, M E MacDonald
Nov 24, 2001·Brain Research Bulletin·V CalabresiC Jodice
Jul 1, 1995·Journal of Biomolecular NMR·C BartelsK Wüthrich

❮ Previous
Next ❯

Citations

Jun 2, 2010·Acta Neuropathologica·Sarah L Hands, Andreas Wyttenbach
Apr 18, 2008·The Cerebellum·Olaf RiessLudger Schöls
Jun 23, 2004·Neurobiology of Disease·Andrej Michalik, Christine Van Broeckhoven
Mar 21, 2007·Nature Structural & Molecular Biology·Yoshitaka NagaiTatsushi Toda
Apr 24, 2007·Nature Structural & Molecular Biology·Pingwei LiPamela J Bjorkman
Oct 19, 2007·The Journal of Biological Chemistry·Zoya IgnatovaLila M Gierasch
Apr 10, 2008·The Journal of Biological Chemistry·Gábor CzirjákPéter Enyedi
Nov 7, 2008·The Journal of Biological Chemistry·Erwann RousseauAnne Bertolotti
Feb 8, 2011·Journal of Biomolecular Structure & Dynamics·Zheng-Li ZhouYih Ho
Sep 25, 2003·Human Molecular Genetics·Andrej Michalik, Christine Van Broeckhoven
Jul 11, 2009·Protein Engineering, Design & Selection : PEDS·Helen M Saunders, Stephen P Bottomley
Mar 29, 2011·Cold Spring Harbor Perspectives in Biology·Steven Finkbeiner
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Margherita RuoppoloCarla Esposito
Jul 22, 2004·European Journal of Biochemistry·Mario AlbrechtThomas Lengauer
Jun 6, 2012·PLoS Biology·Rubén HervásMariano Carrión-Vázquez
May 6, 2010·PLoS Computational Biology·Vinal V LakhaniNikolay V Dokholyan
Aug 13, 2013·PLoS Genetics·Rajesh P MenonPaola Giunti
May 15, 2009·PloS One·Swasti RaychaudhuriDebashis Mukhopadhyay
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Scott L CrickRohit V Pappu
Jan 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher A RossMario Amzel
Nov 22, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ashwani K Thakur, Ronald Wetzel
Apr 16, 2014·Protein Expression and Purification·Ikechukwu AchilonuHeini W Dirr
Nov 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Martin G BurkeS N Yaliraki
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Melanie J BennettPamela J Bjorkman
Aug 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Songming ChenRonald Wetzel
Feb 15, 2016·Neuropeptides·Mohammed InayathullahJayakumar Rajadas
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·Chen ZhangNancy W Karuri
Feb 7, 2012·Journal of Molecular Biology·Ronald Wetzel
Oct 3, 2009·Trends in Cell Biology·Anna von Mikecz
Jun 17, 2008·Protein Science : a Publication of the Protein Society·Chu Kong LiewJoel P Mackay
Mar 24, 2016·Mechanisms of Ageing and Development·Bahareh EftekharzadehSusanne Wegmann
Feb 22, 2011·Proteins·Jyothi L DigambaranathJohn M Finke
Dec 3, 2005·Journal of Molecular Biology·Anusri BhattacharyyaRonald Wetzel
Nov 22, 2005·Proteins·Xiaoling WangRohit V Pappu
Sep 4, 2015·Biophysical Journal·Aaron M Fluitt, Juan J de Pablo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Melanie J BennettPamela J Bjorkman
Proceedings of the National Academy of Sciences of the United States of America
Songming ChenRonald Wetzel
Proceedings of the National Academy of Sciences of the United States of America
A E Bevivino, P J Loll
© 2022 Meta ULC. All rights reserved