Solution structure of the motile major sperm protein (MSP) of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation

Journal of Molecular Biology
A HaafD Neuhaus

Abstract

The major sperm protein (MSP) of Ascaris suum mediates amoeboid motility by forming an extensive intermeshed system of cytoskeletal filaments analogous to that formed by actin in many other amoeboid cells. MSP is a dimeric molecule that polymerizes to form non-polar filaments constructed from two helical subfilaments that wind round one another. Moreover, MSP filaments can interact with one another to form higher-order assemblies without requiring the range of accessory proteins usually employed in actin-based systems. A knowledge of how MSP polymerizes and forms the hierarchical series of helical MSP macromolecular assemblies is fundamental to understanding locomotion in these cells. Here we describe the solution structure of MSP dimers determined by NMR spectroscopy under conditions where MSP does not polymerize to form filaments. The solution structure is indistinguishable from that observed in putative MSP subfilament helices by X-ray crystallography, indicating that MSP polymerization is not accompanied by a major conformational change. We also show that the rate of MSP polymerization associated with movement of vesicles in an in vitro motility assay is enhanced by the presence of magnesium and manganese ions and use NMR t...Continue Reading

References

Oct 1, 1992·Protein Science : a Publication of the Protein Society·R Diamond
Jan 1, 1990·Methods in Enzymology·F W StudierJ W Dubendorff
Jan 1, 1990·Cell Motility and the Cytoskeleton·S Sepsenwol, S J Taft
Feb 1, 1995·Current Opinion in Cell Biology·T M Roberts, M Stewart
Jan 1, 1994·Cell Motility and the Cytoskeleton·K L KingT S Moerland
Jan 12, 1996·Cell·J A Theriot
Jan 1, 1995·Annual Review of Cell and Developmental Biology·J A Theriot
Jul 12, 1996·Journal of Molecular Biology·A HaafM Stewart
May 1, 1996·Journal of Structural Biology·T L BullockM Stewart
Oct 25, 1996·Journal of Molecular Biology·T L BullockM Stewart
Mar 4, 1998·Current Opinion in Cell Biology·H C Joshi
Mar 21, 1998·Nature Structural Biology·T L BullockM Stewart
Nov 1, 1993·Trends in Cell Biology·L M Machesky, T D Poland
Mar 1, 1995·Acta Crystallographica. Section D, Biological Crystallography·R Diamond
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Aug 22, 2007·Trends in Cell Biology·T M Roberts, M Stewart
Jun 1, 1995·Journal of Biomolecular NMR·R H FolmerK Hallenga

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Citations

Nov 21, 2007·Journal of Cellular Biochemistry·Antonio del Castillo-Olivares, Harold E Smith
Nov 23, 2017·G3 : Genes - Genomes - Genetics·Katja R Kasimatis, Patrick C Phillips
Oct 8, 1999·Journal of Molecular Biology·M HelgstrandT Härd
May 13, 2008·Trends in Cell Biology·Sima LevNili Dahan

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