PMID: 8604324Feb 15, 1996Paper

Solution structures of the individual single strands of the fragile X DNA triplets (GCC)n.(GGC)n

Nucleic Acids Research
S V MariappanG Gupta

Abstract

Three-dimensional structures of the fragile X triplet repeats (GCC)n and (GGC)n are derived by using one- dimensional/two-dimensional NMR. Under a wide range of solution conditions (10-150 mM NaCl,pH6-7)(GCC)5-7 strands form exclusively slipped hairpins with a 3' overhanging C. The slipped hairpins of (GCC)n strands show the following structural characteristics: (i) maximization of Watson-Crick G.C pairs; (ii) formation of C.C mispairs at the CpG steps in the stem; (iii) C2'-endo, anti conformations for all the nucleotides. The ability of (GCC)n strands to form hairpin structures more readily than complementary (GGC)n strands suggests preferential slippage during replication and subsequent expansion of the (GCC)n strands. In addition, the C.C. mispairs at the CpG site of (GCC)n hairpins account for their exceptional substrate efficiencies for human methyltransferase. Gel electrophoresis data show that (GGC)n strands form both hairpin and mismatched duplex structures in 10-150 mM NaCl (ph 6-7) for n < 10, but for n > or + 11 hairpin structures are exclusively present. However, (GGC)n strands remain predominantly in the duplex state for n=4-11 under NMR solution conditions, which require DNA concentrations 100- to 1000-fold highe...Continue Reading

References

Dec 1, 1992·Current Opinion in Biotechnology·R R Sinden, R D Wells
May 18, 1992·Science·C T CaskeyD L Nelson
Jan 1, 1989·European Biophysics Journal : EBJ·A N Lane, M J Forster
Jan 1, 1989·International Journal of Radiation Oncology, Biology, Physics·B C WenJ F Doornbos
May 24, 1985·Nucleic Acids Research·D E WemmerB R Reid
Jan 1, 1982·Annual Review of Biochemistry·S B Zimmerman
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·X ChenG Gupta
Feb 1, 1994·Nature Genetics·R I Richards, G R Sutherland
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·M Fry, L A Loeb
Nov 1, 1993·Current Biology : CB·Y TrottierJ L Mandel
Apr 1, 1986·Journal of Computational Chemistry·Scott J WeinerDavid A Case

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Citations

Aug 1, 1997·Electrophoresis·A J JeffreysJ Yardley
Jun 1, 1997·Current Opinion in Structural Biology·A Lebrun, R Lavery
Jul 17, 1998·Current Opinion in Structural Biology·C E Pearson, R R Sinden
Mar 26, 1998·Journal of Biomolecular Structure & Dynamics·S V MariappanG Gupta
Mar 26, 1998·Journal of Biomolecular Structure & Dynamics·X ChenG Gupta
Aug 1, 1998·Nucleic Acids Research·H ArthanariP H Bolton
Apr 11, 2000·Nucleic Acids Research·V M Marathias, P H Bolton
Nov 20, 2001·Nucleic Acids Research·P Fojtík, M Vorlícková
Jan 14, 2004·Nucleic Acids Research·Petr FojtíkMichaela Vorlícková
Jan 24, 2012·Biology Direct·Harold S Bernhardt, Warren P Tate
Nov 16, 2006·PLoS Biology·Jennifer E PoseyDavid B Roth
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·J VölkerK J Breslauer
Jan 15, 2014·Human Molecular Genetics·Dmitry YudkinKaren Usdin
Nov 26, 2008·Biochimie·Daniel RenciukMichaela Vorlícková
Jul 19, 2011·Chembiochem : a European Journal of Chemical Biology·Amit KumarMatthew D Disney
Oct 16, 2002·Biochimica Et Biophysica Acta·Kallur Siddaramaiah LathaMysore Ananthamurthy Viswamitra
Jun 15, 1997·Nucleic Acids Research·M Mitas
Nov 4, 2004·Journal of Biotechnology·James R CookeEli Keshavarz-Moore
Mar 17, 2006·Biochemical and Biophysical Research Communications·Gargi Ghosal, K Muniyappa
Feb 28, 2006·Biochemical and Biophysical Research Communications·Mikhail SkoblovAncha Baranova
Feb 6, 1998·Journal of Molecular Biology·J M Darlow, D R Leach
Feb 6, 1998·Journal of Molecular Biology·J M Darlow, D R Leach
Jun 7, 2019·Journal of Biomolecular Structure & Dynamics·Aparna Bansal, Shrikant Kukreti
Jun 22, 2002·The Journal of Biological Chemistry·Philip PinheiroJames A McClellan
Jan 13, 2021·Journal of Chemical Theory and Computation·Thomas J OweidaYaroslava G Yingling

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