Solution study of the Escherichia coli DNA polymerase III clamp loader reveals the location of the dynamic ψχ heterodimer

Structural Dynamics
Farzaneh TondnevisRobert McKenna

Abstract

Several X-ray crystal structures of the E. coli core clamp loader containing the five core (δ', δ, and three truncated γ) subunits have been determined, but they lack the ψ and χ subunits. We report the first solution structure of the complete seven-subunit clamp loader complex using small angle X-ray scattering. This structure not only provides information about the location of the χ and ψ subunits but also provides a model of the dynamic nature of the clamp loader complex.

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Citations

Mar 13, 2016·Journal of Structural Biology·Farzaneh TondnevisRobert McKenna

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Methods Mentioned

BETA
X-ray
crosslinking studies

Software Mentioned

GNOM
CYROSL
RAW
CHIMERA
COOT
DAMAVER
SASPLOT
PRIMUS
Robetta
CRYSOL

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