Solution (sup13)C Nuclear Magnetic Resonance Spectroscopic Analysis of the Amino Acids of Methanosphaera stadtmanae: Biosynthesis and Origin of One-Carbon Units from Acetate and Carbon Dioxide.

Applied and Environmental Microbiology
T L MillerS Bank

Abstract

We found that general pathways for amino acid synthesis of Methanosphaera stadtmanae, a methanogen that forms CH(inf4) from H(inf2) and methanol, resembled those of methanogens that form CH(inf4) from CO(inf2) or from the methyl group of acetate. We determined the incorporation of (sup14)C-labeled CO(inf2), formate, methanol, methionine, serine, and acetate into cell macromolecules. Labeling of amino acid carbons was determined by solution nuclear magnetic resonance spectroscopy after growth with (sup13)C-labeled acetate, CO(inf2), serine, and methanol. The (alpha) and (beta) carbons of serine and alanine were formed from carboxyl and methyl carbons of acetate, respectively, and the amino acid carboxyl groups were formed from CO(inf2). This indicates that pyruvate was formed by reductive carboxylation of acetate. Labeling of the methyl carbon of methionine indicated that the major route of synthesis was from the hydroxymethyl carbon of serine that arises from the methyl carbon of acetate. Methanol was a minor source of the methyl of methionine. Unambiguous assignment was made of the sources of all carbons of histidine. Labeling of the histidine 7 position ((epsilon) carbon) was consistent with formation from the C-2 of the puri...Continue Reading

References

May 15, 1991·FEMS Microbiology Letters·W M van de WijngaardC van der Drift
Mar 1, 1987·Microbiological Reviews·W J JonesW B Whitman
Nov 1, 1988·Applied and Environmental Microbiology·S F LajoieM J Wolin
Jun 1, 1985·Journal of Bacteriology·I EkielG B Patel
Jul 1, 1985·Journal of Bacteriology·W J JonesR S Wolfe
Apr 1, 1993·Applied and Environmental Microbiology·G D SprottG B Patel

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Citations

Oct 31, 2006·Journal of Bacteriology·Sunna HelgadóttirDavid E Graham
Feb 21, 2019·Organic & Biomolecular Chemistry·Fayrouz El MaddahGabriele M König
Dec 1, 1996·Solid State Nuclear Magnetic Resonance·S BankT L Miller

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