Solvent-Augmented Mineralization of Pyrene by a Mycobacterium sp.

Applied and Environmental Microbiology
I Y Jimenez, R Bartha

Abstract

The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain, morphology, and fatty acid profile identified it as a Mycobacterium sp. In a mineral salts solution, the isolate mineralized 50% of a 250-(mu)g/ml concentration of [(sup14)C]pyrene in 2 to 3 days. Detergent below the critical micelle concentration increased the pyrene mineralization rate to 154%, but above the critical micelle concentration, the detergent severely inhibited pyrene mineralization. The water-miscible solvent polyethylene glycol was inhibitory. The hydrophobic solvents heptamethylnonane, decalin, phenyldecane, and diphenylmethane were also inhibitory at several concentrations tested, but the addition of paraffin oil, squalene, squalane, tridecylcyclohexane, and cis-9-tricosene at 0.8% (vol/vol) doubled pyrene mineralization rates by the Mycobacterium sp. without being utilized themselves. The Mycobacterium sp. was found to have high cell surface hydrophobicity and adhered to the emulsified solvent dr...Continue Reading

References

Jan 1, 1990·Applied Microbiology and Biotechnology·W D WeissenfelsJ Klein
Oct 1, 1988·Applied and Environmental Microbiology·M A HeitkampC E Cerniglia
Jun 1, 1988·Applied and Environmental Microbiology·M A Heitkamp, C E Cerniglia
Jun 1, 1993·Applied and Environmental Microbiology·B BoldrinC Fritzsche
Mar 1, 1977·Applied and Environmental Microbiology·R S Wodzinski, D Larocca
Nov 1, 1979·Applied and Environmental Microbiology·A C Marinucci, R Bartha
May 1, 1991·Applied and Environmental Microbiology·R A Efroymson, M Alexander

❮ Previous
Next ❯

Citations

Mar 29, 2000·Journal of Bacteriology·R A Kanaly, S Harayama
Jan 6, 2009·Journal of Applied Microbiology·C O ObuekweE S Al-Saleh
May 15, 2009·International Journal of Environmental Research and Public Health·Jong-Su SeoQing X Li
Oct 1, 1999·Environmental Toxicology and Chemistry·Robert A Kanaly, Richard Bartha
Sep 30, 2000·Applied and Environmental Microbiology·R A KanalyS Harayama
Apr 3, 2001·Applied and Environmental Microbiology·J D MoodyC E Cerniglia
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jonathan D Van HammeOwen P Ward
Apr 25, 2001·Applied and Environmental Microbiology·P Y Cheung, B K Kinkle
Jul 19, 2003·Environmental Technology·J H Berg Schuur, B Mattiasson
Mar 11, 2005·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Jennifer Paige HarperMelinda M Lalor
Mar 16, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Alok K PandeyLata Nain
May 2, 2000·Applied and Environmental Microbiology·L BastiaensL Diels
Dec 8, 2009·Current Microbiology·Oluwafemi S ObayoriOlukayode O Amund
Apr 15, 2010·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Yanjiao ChenMing Hong Wong
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·Ester HeathMichael P Bratty
Nov 26, 2003·FEMS Microbiology Letters·Philip H LeeKirk T Semple
Sep 12, 1998·Chemosphere·K RehmannA A Kettrup
Mar 15, 2006·Applied and Environmental Microbiology·R KanalyM Findlay
Sep 3, 2010·Environmental Science & Technology·Albert D VenosaMakram T Suidan
Mar 4, 2008·Microbial Cell Factories·Ireri V Robles-GonzálezHéctor M Poggi-Varaldo
Feb 5, 2010·Journal of Colloid and Interface Science·Caroline Warne ZouekiSubhasis Ghoshal

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.