PMID: 9165531May 1, 1997Paper

Solvent effects on the solubility and physical stability of human insulin-like growth factor I

Pharmaceutical Research
J R FranssonE Florin-Robertsson

Abstract

The solubility and physical stability of human Insulin-like Growth Factor I (hIGF-I) were studied in aqueous solutions with different excipients. The solubility of hIGF-I was determined by UV-absorption and quantification of light blocking particles. The physical stability of hIGF-I was studied with differential scanning calorimetry (DSC) and circular dichroism (CD) spectroscopy. Human IGF-I precipitated at low temperature in the presence of 140 mM benzyl alcohol and 145 mM sodium chloride. CD data showed that the tertiary structure of hIGF-I during these conditions was perturbed compared to that in 5 mM phosphate buffer. In the presence of benzyl alcohol 290 mM mannitol stabilized hIGF-I. Sodium chloride or mannitol by themselves had no effect on either the solubility or the tertiary structure. Benzyl alcohol was attracted to hIGF-I, whereas sodium chloride was preferentially excluded. The attraction of benzyl alcohol was reinforced by sodium chloride leading to salting-out of hIGF-I. The CD-data indicated interactions of benzyl alcohol with phenylalanine in hIGF-I. Thermal denaturation of hIGF-I occurred in all solutions with sodium chloride, whereas mannitol or benzyl alcohol had no effect on the thermal stability. The therm...Continue Reading

Citations

Mar 19, 2005·Drug Development and Industrial Pharmacy·Sitaram P Velega, Johan Carlfors
Mar 31, 2009·Tissue Engineering. Part C, Methods·Ashwin NairLiping Tang
May 21, 2005·Pharmaceutical Research·Muppalla SukumarMichael R De Felippis
Sep 28, 2012·Journal of Biomedical Materials Research. Part a·Roman A Perez, Hae-Won Kim
May 19, 2011·The Journal of Pharmacy and Pharmacology·José A Rodríguez-MartínezKai Griebenow
Nov 19, 2003·Journal of Biomaterials Applications·C CarrascosaJ J Torrado
Oct 2, 2007·Biophysical Chemistry·Tsutomu ArakawaSerge N Timasheff
Jun 19, 2008·Vascular Pharmacology·Kurt Ming-Chao LinSubbiah Rajasekaran
Oct 22, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Monu Kumari GoyalArvind Kumar Bansal
Mar 24, 2010·Journal of Pharmaceutical Sciences·Monu Kumari GoyalArvind Kumar Bansal

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