Solvent hydrolysis rate determines critical quality attributes of PLGA microspheres prepared using non-volatile green solvent

Journal of Biomaterials Science. Polymer Edition
H KimHongkee Sah

Abstract

This study aimed to develop a solvent hydrolysis-based microencapsulation technique that could fabricate PLGA microspheres, while using dimethyl carbonate as a green dispersed solvent. Instead of existing physical solvent removal techniques, a strategy was derived to use a chemical reaction that could transform oil droplets into microspheres. An oil-in-water emulsion was first produced by emulsifying a PLGA/Nile red/progesterone/dimethyl carbonate dispersed phase in an aqueous phase. Adding a NaOH solution into the emulsion led to the decomposition of dimethyl carbonate that partitioned to the water phase. This chemical reaction allowed the continuous diffusion of dimethyl carbonate existing in emulsion droplets into the aqueous phase and its complete removal. The solvent hydrolysis rate was identified as the most important process parameter affecting the major quality attributes of PLGA microspheres. For instance, it was shown through a 3D analysis that Nile red was uniformly dispersed across the microsphere matrix at a fast solvent hydrolysis rate. In comparison, a slow solvent hydrolysis rate allowed the dye to disperse heterogeneously in the microsphere matrix. A drug crystallization phenomenon, being commonly observed in c...Continue Reading

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Citations

Jul 24, 2020·International Journal of Cosmetic Science·Emilie MunnierFranck Bonnier

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Methods Mentioned

BETA
Fluorescence

Software Mentioned

TA Universal Analysis 2000
ApoTom

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