PMID: 20127049Feb 4, 2010Paper

Solvent-induced virus inactivation by acidic arginine solution

International Journal of Molecular Medicine
Kazuko TsujimotoTsutomu Arakawa

Abstract

Viral clearance is a primary concern for parenteral protein biopharmaceuticals. Low pH, detergent/solvent wash, or heating, called pasteurization, has been the main process for virus inactivation. Detergent/solvent wash is also used to treat superficial infectious diseases, including herpes simplex virus (HSV) infections. Herein we examined virus inactivation effects of acidic arginine on HSV type 2 (HSV-2) as a function of pH and temperature in an attempt to find solvent conditions that are effective for virus inactivation, yet are compatible with in vivo applications. Aqueous arginine at 0.7 M was highly effective on HSV-2, more so at lower pH and higher temperature. Its effects were stronger than 0.1 M citrate, 0.1 M citrate/0.6 M NaCl or 0.7 M citrate at any pH and temperature. This demonstrates that strong virus inactivation effects of arginine are not simply due to ionic strength or high concentration and arginine possesses a unique property that results in irreversible damage in virus particles. Such strong virus inactivation effects can be used in vivo for certain superficial infectious diseases, such as genital infections.

Citations

Feb 9, 2012·Advances in Virology·Hisashi YamasakiA Hajime Koyama
Oct 15, 2014·Advanced Drug Delivery Reviews·José L TlaxcaRichard L Remmele
Jun 2, 2016·PloS One·Aleksandra MilewskaKrzysztof Pyrc
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Satoshi OhtakeA Hajime Koyama
Sep 3, 2019·Japanese Journal of Infectious Diseases·Keiko IkedaA Hajime Koyama
Oct 18, 2019·Biotechnology Progress·Christa Meingast, Caryn L Heldt

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