Solvent separating secondary metabolites directly from biosynthetic tissue for surface-assisted laser desorption ionisation mass spectrometry

Marine Drugs
David RuddN H Voelcker

Abstract

Marine bioactive metabolites are often heterogeneously expressed in tissues both spatially and over time. Therefore, traditional solvent extraction methods benefit from an understanding of the in situ sites of biosynthesis and storage to deal with heterogeneity and maximize yield. Recently, surface-assisted mass spectrometry (MS) methods namely nanostructure-assisted laser desorption ionisation (NALDI) and desorption ionisation on porous silicon (DIOS) surfaces have been developed to enable the direct detection of low molecular weight metabolites. Since direct tissue NALDI-MS or DIOS-MS produce complex spectra due to the wide variety of other metabolites and fragments present in the low mass range, we report here the use of "on surface" solvent separation directly from mollusc tissue onto nanostructured surfaces for MS analysis, as a mechanism for simplifying data annotation and detecting possible artefacts from compound delocalization during the preparative steps. Water, ethanol, chloroform and hexane selectively extracted a range of choline esters, brominated indoles and lipids from Dicathais orbita hypobranchial tissue imprints. These compounds could be quantified on the nanostructured surfaces by comparison to standard curv...Continue Reading

References

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Citations

Dec 9, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roselyn Valles-ReginoKirsten Benkendorff
May 11, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Ying-Ning HoYu-Liang Yang
Mar 4, 2020·BMC Complementary Medicine and Therapies·Shanu HodaPooja Vijayaraghavan
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep

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