Solvent viscosity mismatch between the solute plug and the mobile phase: considerations in the applications of two-dimensional HPLC

The Analyst
R Andrew Shalliker, Georges Guiochon

Abstract

Understanding the nature of viscosity contrast induced flow instabilities is an important aspect in the design of two-dimensional HPLC separations. When the viscosity contrast between the sample plug and the mobile phase is sufficiently large, the phenomenon known as viscous fingering can be induced. Viscous fingering is a flow instability phenomenon that occurs at the interface between two fluids with different viscosities. In liquid chromatography, viscous fingering results in the solute band undergoing a change in form as it enters into the chromatography column. Moreover, even in the absence of viscous fingering, band shapes change shape at low viscosity contrasts. These changes can result in a noticeable change in separation performance, with the result depending on whether the solvent pushing the solute plug has a higher or lower viscosity than the solute plug. These viscosity induced changes become more important as the solute injection volume increases and hence understanding the process becomes critical in the implementation of multidimensional HPLC techniques, since in these techniques the sample injection plug into the second dimension is an order of magnitude greater than in one-dimensional HPLC. This review article...Continue Reading

References

Aug 19, 2000·Journal of Chromatography. a·R A ShallikerG Guiochon
Jul 13, 2005·Journal of Chromatography. a·Kirsty J MayfieldGeorges Guiochon
Apr 20, 2006·Journal of Chromatography. a·Heather J CatchpooleGeorges Guiochon
Oct 13, 2006·Journal of Chromatography. a·R Andrew ShallikerGeorges Guiochon
Apr 11, 2007·Journal of Chromatography. a·G RousseauxM Martin
Jul 14, 2007·Journal of Chromatography. a·R A ShallikerG Guiochon
Mar 14, 2008·Journal of Chromatography. a·Georges GuiochonR Andrew Shalliker

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Citations

Oct 23, 2010·Analytical and Bioanalytical Chemistry·Marianne Gaborieau, Patrice Castignolles
May 22, 2013·Journal of Chromatography. a·Manoranjan MishraMichel Martin
Oct 19, 2011·The Analyst·Michelle CamenzuliR Andrew Shalliker
Oct 19, 2011·Journal of Chromatography. a·G RousseauxA De Wit
Oct 26, 2010·Journal of Separation Science·Patrice Castignolles, Marianne Gaborieau
Mar 5, 2015·Journal of Separation Science·Danielle N BassanesePaul G Stevenson
Mar 19, 2014·Journal of Chromatography. a·Paul G StevensonNeil W Barnett
Nov 26, 2016·Journal of Separation Science·Petr Česla, Jana Křenková

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