PMID: 334537Sep 1, 1977

Somatic antigens of Shigella. The strucuture of the specific polysaccharide chain of Shigella dysenteriae type 5 lipopolysaccharide

European Journal of Biochemistry
B A DmitrievN K Kochetkov

Abstract

The specific polysaccharide was released from Shigella dysenteriae type 5 lipopolysaccharide by mild acidic hydrolysis and then purified by gel chromatography on Sephadex G-50. The polysaccharide was built up of residues of D-mannose, 2-acetamido-2-deoxy-D-glucose, 3-0-(D-1-carboxyethyl)-L-rhamnose (rhamnolactylic acid) and 0-acetyl groups in a ratio 2:1:1:1. On the basis of radiospectroscopy, methylation analysis, Smith degradation, and chromium trioxide oxidation, the repeating oligosaccharide unit of the polysaccharide can be assigned the following structure: (formula: see text) where GlcNAc is 2-acetamido-2-deoxy-D-glucopyranose, Manp is mannopyranose, RhaLcA is rhammolacytic acid and Ac is an acetyl group. The serological properties of Sh. dysenteriae somatic antigens are discussed in relation to the chemical structures of their specific polysaccharides.

Citations

Jan 15, 1975·European Journal of Biochemistry·B A DmitrievI L Hofman
Jul 1, 1970·European Journal of Biochemistry·B JannF Orskov
Dec 17, 1973·European Journal of Biochemistry·B A DmitrievI L Hofman

Related Concepts

Chromium product (pharmacologic preparation)
Sephadex G
In Vivo NMR Spectroscopy
Protein Methylation
Chlorozotocin
Dysentery, Bacillary
Mannose
Oxidation
Polysaccharides, Bacterial
Mass Spectrometry

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