Somatic cell cloning without micromanipulators

Cloning
G VajtaA O Trounson

Abstract

Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst rates in the three systems tested for zona-free embryo culture were 0%, 18%, and 36% for microdrops, well of the wells (WOW system), and microcapillaries (GO system), respectively. This simple, rapid, and inexpensive procedure may become a useful alternative to the existing techniques for somatic cell nuclear transfer for large-scale application of the technology.

References

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Citations

Jan 22, 2008·Journal of Assisted Reproduction and Genetics·Lleretny RodríguezFidel Ovidio Castro
Jul 5, 2013·In Vitro Cellular & Developmental Biology. Animal·S M HosseiniM H Nasr-Esfahani
Jul 13, 2013·In Vitro Cellular & Developmental Biology. Animal·Lleretny Rodríguez-AlvarezFidel Ovidio Castro
Apr 20, 2005·Molecular and Cellular Endocrinology·Hanne G PedersenTorben Greve
Dec 25, 2002·Theriogenology·C GalliG Lazzari
Apr 29, 2009·Zygote : the Biology of Gametes and Early Embryos·Yongsheng WangYong Zhang
Jun 9, 2009·Zygote : the Biology of Gametes and Early Embryos·Liliana ChacónC Earle Pope
Feb 27, 2004·Reproduction, Fertility, and Development·R T TecirliogluA O Trounson
Jan 30, 2007·Reproduction, Fertility, and Development·Gábor VajtaZoltán Macháty
May 2, 2003·Cloning and Stem Cells·Ian Wilmut
Apr 26, 2003·Cloning and Stem Cells·B ObackD N Wells
Aug 22, 2003·Cloning and Stem Cells·P HolmH Callesen
Jan 22, 2004·Cloning and Stem Cells·Cesare GalliGiovanna Lazzari
Jan 22, 2004·Cloning and Stem Cells·B Oback, D N Wells
Jan 22, 2004·Cloning and Stem Cells·Teija T Peura, Gábor Vajta
Apr 27, 2004·Cloning and Stem Cells·Guang-Peng LiThomas D Bunch
Apr 9, 2011·Cellular Reprogramming·M H Nasr-EsfahaniH Vojgani
Jun 24, 2005·Cloning and Stem Cells·R RibasI Wilmut
Mar 31, 2006·Cloning and Stem Cells·R RibasI Wilmut
Jan 2, 2007·Cloning and Stem Cells·J LiG Vajta
Apr 21, 2007·The Journal of Reproduction and Development·Ralf PoehlandSanjay Bhojwani
Feb 26, 2013·PloS One·Peng ZhangRunlin Z Ma
May 19, 2011·Yi chuan = Hereditas·Peng ZhangRun-Lin Ma
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Irina LagutinaCesare Galli
Sep 13, 2007·Stem Cell Reviews·R Tayfur TecirliogluAlan O Trounson
May 15, 2010·Reproductive Biomedicine Online·Gábor VajtaBarry D Bavister
Mar 6, 2010·Reproductive Biomedicine Online·Gábor VajtaJohn Yovich
Apr 17, 2007·Trends in Biotechnology·Gábor Vajta
Mar 16, 2007·Cryobiology·Y DuG Vajta

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