PMID: 2109325Apr 1, 1990Paper

Somatic diversification of chicken immunoglobulin light chains by point mutations

Proceedings of the National Academy of Sciences of the United States of America
R ParvariI Schechter

Abstract

The light-chain locus of chicken has 1 functional V lambda 1 gene, 1 J gene, and 25 pseudo-V lambda-genes (where V = variable and J = joining). A major problem is which somatic mechanisms expand this extremely limited germ-line information to generate many different antibodies. Weill's group [Reynaud, C. A., Anquez, V., Grimal, H. & Weill, J. C. (1987) Cell 48, 379-388] has shown that the pseudo-V lambda-genes diversify the rearranged V lambda 1 by gene conversion. Here we demonstrate that chicken light chains are further diversified by somatic point mutations and by V lambda 1-J flexible joining. Somatic point mutations were identified in the J and 3' noncoding DNA of rearranged light-chain genes of chicken. These regions were analyzed because point mutations in V lambda 1 are obscured by gene conversion; the J and 3' noncoding DNA are presented in one copy per haploid genome and are not subject to gene conversion. In rodents point mutations occur as frequently in the V-J coding regions as in the adjacent flanking DNA. Therefore, we conclude that somatic point mutations diversify the V lambda 1 of chicken. The frequency (0-1%) and distribution of the mutations (decreasing in number with increased distance from the V lambda 1 s...Continue Reading

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Citations

Feb 1, 1991·Current Opinion in Immunology
Jun 7, 2005·Molecular Immunology·Tania Romo-González, Enrique Vargas-Madrazo
Jun 8, 2001·Veterinary Immunology and Immunopathology·J IvánJ Mészáros
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Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·N MotoyamaT Azuma
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Jan 1, 1995·ILAR Journal·W. Carey HanlyB. Taylor Bennett
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Jun 16, 2004·BMC Immunology·Thomas M Conlon, Kerstin B Meyer
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Dec 1, 1992·Trends in Genetics : TIG·C B Thompson
Jan 20, 2010·Veterinary Immunology and Immunopathology·Wilmar Dias da Silva, Denise V Tambourgi
Sep 25, 2016·Applied and Environmental Microbiology·Chi-Hsin LeeYi-Yuan Yang
May 20, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matthieu BascoveJean-Pol Frippiat
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Dec 8, 2020·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Chi-Hsin LeeYi-Yuan Yang

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