Somatic embryogenesis from leaf tissues of macaw palm [Acrocomia aculeata (Jacq.) Lodd. ex Mart

Anais Da Academia Brasileira De Ciências
Filipe S MeiraJonny E Scherwinski-Pereira

Abstract

A somatic embryogenesis protocol was developed from the immature leaves of adult plants of the macaw palm. Leaf explants from different regions of the palm heart were used for callus initiation in a modified Y3 medium, supplemented with 2,4-D or Picloram at 450 μM. Calli were separated from the leaf explants at 6-, 9- and 12-month periods and transferred to a fresh culture medium of the same composition. They were multiplied for up to 120 days. Reduced concentrations of 2,4-D and Picloram were used to differentiate somatic embryos. They were then germinated in a medium without plant growth regulators. Morphological and anatomical analyses were conducted at different stages of the embryogenic process. The best results for callus induction were achieved by Picloram, when explants were maintained for up to 9 months on culture medium (64.9%). The farthest portions of the apical meristem were those that provided the biggest calli formation. The formation of the somatic embryos was observed from the calli multiplication phase. Reduction in concentrations of growth regulators failed to promote the formation of complete plants. Picloram at 450 μM promotes high callogenesis in leaf tissues of macaw palm, with a potential for somatic emb...Continue Reading

References

Apr 1, 1992·The Plant Cell·A J De JongS C De Vries
Jan 12, 2001·Plant Physiology·A M Jones
Dec 12, 2012·American Journal of Botany·P Barry Tomlinson, Brett A Huggett
Nov 26, 2013·Anais Da Academia Brasileira De Ciências·Marlúcia S PáduaVanessa C Stein

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