PMID: 9174166May 1, 1997Paper

Somatic transgene immunization with DNA encoding an immunoglobulin heavy chain

DNA and Cell Biology
Mara GerloniMaurizio Zanetti

Abstract

A plasmid DNA containing a chimeric immunoglobulin heavy-chain gene with tissue-specific promoter and enhancer elements was used as a model system to study the events triggered by a single intraspleen DNA inoculation in adult C57Bl/6 mice. A single intraspleen inoculation was followed in a week by secretion of transgene immunoglobulins and production of immunoglobulin M (IgM) anti-immunoglobulins. Their kinetics of serum appearance were almost superimposable. While anti-immunoglobulin antibodies remained detectable for over 6 months, transgene immunoglobulins disappeared after 3-4 weeks. However, transgene mRNA was detected in the spleen for 4 months. A multiplex polymerase chain reaction (PCR) analysis on splenic genomic DNA 17 days after inoculation demonstrated that the transgene was integrated in the host chromosomal DNA. The nucleotide sequence of the rearranged VDJ region from splenic genomic DNA was identical to that of the parental plasmid DNA, hence ruling out that hypermutation had occurred. A booster injection of immunoglobulin encoded by the transgene on day 200 elicited a typical secondary immune response with IgG1 and IgG2b antibodies. These results demonstrate that a single inoculation of an immunoglobulin heavy-...Continue Reading

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Citations

Oct 11, 2005·Autoimmunity Reviews·Maurizio Zanetti
Nov 5, 1997·Nature Biotechnology·J B Ulmer
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·M GerloniM Zanetti
Jun 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·S XiongM Zanetti
Jun 14, 2001·DNA and Cell Biology·A CanoK Manoutcharian
Mar 14, 2002·International Reviews of Immunology·M Gerloni, M Zanetti
Jul 6, 2004·Immunological Reviews·Maurizio ZanettiMara Gerloni
Nov 25, 2003·Hematology·Helen E HeslopJeffrey J Molldrem
Sep 24, 2016·Oncotarget·Viswa Teja Colluru, Douglas G McNeel
Aug 24, 2002·Scandinavian Journal of Immunology·P CastiglioniM Zanetti
Oct 29, 1998·FEMS Immunology and Medical Microbiology·S ContiL Polonelli

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