PMID: 6111581Feb 1, 1981Paper

Somatostatin binding factor from chicken pancreas

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
F DielH J Quabbe

Abstract

A soluble somatostatin binding factor was detected in cell-free extracts from chicken pancreas. For binding measurements Tyr1-somatostatin was radio-labeled with 125I by the lactoperoxidase technique. Specific radioactivity of about 18.5 MBq/nmol was achieved. Maximal total binding is approximately 0.17 (B/T) in the presence of 30 mg/l pancreatic protein. The specific binding is 0.10 and is suppressed by addition of 1 mg/l synthetic cold cyclic somatostatin. The dose-response curve of synthetic cyclic somatostatin is in the range of 0.6-600 nmol/l. Ca2+ and reduced thiol-reagents inhibit the specific binding. Insulin, glucagon and corticotropin show a low, and luliberin and reduced somatostatin a high cross-reactivity. Molecular weight was estimated by gel filtration and the specific binding molecule was eluted at a Kav = 0.2 on an Ultrogel (AcA 54) column. This corresponds to Mr 40 000. Electrophoretic properties of the binding complex and semipurification by polyacrylamide disc gel electrophoresis: relative mobility of the 125I-Tyr-somatostatin binding complex is about 0.6. Relative mobilities of binding-protein fractions are 0.71 and 0.74. Highest relative specific binding was detected in the (100 000 g) cytosol fractions. B...Continue Reading

References

Aug 1, 1977·The Biochemical Journal·N OgawaP Brazeau
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Sep 1, 1978·Metabolism: Clinical and Experimental·R EldeR Luft
Aug 9, 1979·The New England Journal of Medicine·G J KrejsR H Unger
Feb 1, 1978·Canadian Journal of Physiology and Pharmacology·N OgawaH G Friesen
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Sep 1, 1975·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·L OrciC Rufener

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Citations

Jun 1, 1982·Regulatory Peptides·L Hilsted, J J Holst
Feb 1, 1986·Regulatory Peptides·B RocaJ C Prieto
Nov 1, 1987·Regulatory Peptides·V BarriosE Arilla
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·M P López-RuizE Arilla
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M P López-RuizJ C Prieto

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