PMID: 7543685Jul 1, 1995Paper

Somatostatin in a water-restricted environment: fluorescence and circular dichroism study in AOT reverse micelles

Photochemistry and Photobiology
K Bhattacharyya, S Basak

Abstract

Steady-state and time-resolved fluorescence emission from the single tryptophan residue of somatostatin, and the kinetics of quenching of this emission, were studied in aqueous solution and in reverse micelles of sodium bis (2-ethylhexyl) sulfosuccinate (AOT)/water/isooctane, a system that mimics the water-membrane interface well. Incorporation into micelles caused blue shifts and reduced band-widths of the emission peaks and altered the quantum yields with respect to emission from bulk water. Steady-state anisotropy values also increased considerably on micellization. These observations point to reduced polarity of the environment around the Trp residue of the peptide, as well as restricted freedom of its rotational motions, due to transfer from the aqueous to the micellar phase. Fluorescence emission kinetics of the Trp moiety followed biexponential decay laws in both aqueous and micellar media. Static and dynamic quenching constants were measured for acrylamide and CCI4 quenchers localized in the micellar and organic pseudophases, respectively, using both steady-state and time-resolved experiments. The efficiency of dynamic quenching by acrylamide became vanishingly small in going from water to reverse micelles, in sharp con...Continue Reading

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Citations

Jun 6, 2003·Chemistry and Physics of Lipids·Eduardo P MeloSteffen B Petersen
Dec 14, 2007·Proteins·Swasti RaychaudhuriNitai P Bhattacharyya
Jun 14, 2005·Biophysical Chemistry·Dilip Kumar DebnathSoumen Basak
Apr 17, 1998·Critical Reviews in Biochemistry and Molecular Biology·M Tuena de Gómez-Puyou, A Gómez-Puyou
Sep 29, 2004·Journal of Biochemical and Molecular Toxicology·Charles A Smith, Channing L Hinman
Apr 15, 2014·Biopolymers·Belén HernándezMahmoud Ghomi

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