PMID: 9419835Jan 7, 1998Paper

Somatostatin plays a dual, stimulatory/inhibitory role in the control of growth hormone secretion by two somatotrope subpopulations from porcine pituitary

Journal of Neuroendocrinology
J L RamírezFrancisco Gracia-Navarro

Abstract

Previous results from our laboratory demonstrated the existence of two subpopulations of porcine somatotropes of low- (LD) and high density (HD) that exhibit differences in ultrastructure and respond in an opposite manner to somatostatin (SRIF) in vitro. In LD cells, SRIF did not affect basal growth hormone (GH) release but partially blocked the stimulatory effect induced by GH-releasing factor (GRF). Conversely, SRIF paradoxically stimulated the secretory activity of HD somatotropes. Here, we have analysed in detail the basic parameters that characterize this differential response. To this end, the time- and dose-dependent effects of SRIF-14 were evaluated on separate monolayer cultures of both subpopulations. Likewise, the direct effect of the peptide on individual somatotropes from each subset was assessed by cell immunoblot assay. Finally, we compared the effects of SRIF-14 and SRIF-28 on cultures of LD and HD cells. SRIF-14 (10(-7) M) induced a rapid (30 min) and sustained (4 h) 2-fold increase in GH release from HD cells, whereas it did not affect GH secretion from LD somatotropes. Surprisingly, a low dose of SRIF (10(-15) M) stimulated GH release from both LD (154.1 +/- 8.2% of basal, P < 0.05) and HD (337.2 +/- 55.5% of...Continue Reading

Citations

Apr 6, 2002·Archives of Physiology and Biochemistry·F Gracia-NavarroE Delgado
May 22, 2009·Annals of the New York Academy of Sciences·Manuel D GaheteJusto P Castaño
Jun 18, 2003·Critical Reviews in Toxicology·Edward J Calabrese, Linda A Baldwin
Dec 6, 2020·Molecular and Cellular Endocrinology·S Thierry OmouessiRichard R Neubig
Apr 28, 2001·Molecular and Cellular Endocrinology·W K Yunker, J P Chang
Apr 4, 2008·Molecular and Cellular Endocrinology·Kyriaki Thermos

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