Somatostatin receptor regulation of gastric enterochromaffin-like cell transformation to gastric carcinoid

Surgery
J F BorinI M Modlin

Abstract

Although somatostatin is recognized as an inhibitor of neuroendocrine cell secretion, its effect on cell proliferation has not been well defined. Generation of low acid and hypergastrinemia through irreversible H2-receptor blockade (loxtidine) in the African rodent mastomys results in gastric carcinoids (ECLomas) within 4 months. This study was undertaken to evaluate and characterize the precise somatostatin receptor (SSTR) subtype on the mastomys enterochromaffin-like (ECL) cell and to define its role in the regulation of ECL cell secretion and proliferation. A pure preparation (approximately 90%) of ECL cells was derived by a combination of pronase digestion and density gradient separation. We assessed the effect of somatostatin (10(-15) to 10(-7) mol/L) on gastrin-stimulated ECL cell histamine secretion and DNA synthesis (bromodeoxyuridine uptake). SSTR2 subtype was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) using gene specific primers and mRNA isolated from normal and hypergastrinemia-induced ECLoma. The polymerase chain reaction product was confirmed by Southern analysis, subcloned, and sequenced. Somatostatin inhibited both gastrin-stimulated histamine secretion (IC50, 5 x 10(-13) mol/L) and DNA...Continue Reading

References

Mar 11, 1991·Annals of Surgery·B M EversJ C Thompson
Apr 21, 1972·Nature·J W BlackE M Parsons
Sep 15, 1994·Annals of the New York Academy of Sciences·I M ModlinC J Soroka
Aug 1, 1995·Neuroscience·T Reisine, G I Bell
Apr 1, 1995·Archives of Surgery·I M ModlinG P Lawton
Jan 1, 1995·European Journal of Endocrinology·J BertheratJ Epelbaum

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Citations

Sep 15, 2012·Current Gastroenterology Reports·Mark Kidd, Bjorn I Gustafsson
Aug 30, 2003·Surgical Oncology·Irvin M ModlinMark Kidd
Dec 31, 2003·Journal of the American College of Surgeons·Irvin M ModlinMark Kidd
Dec 2, 2004·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·I M ModlinA Argiris
Mar 16, 2006·The Journal of Clinical Endocrinology and Metabolism·Irvin M ModlinManish C Champaneria
Feb 4, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·M KiddM C Champaneria

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