Somatostatin regulates tight junction function and composition in human keratinocytes

Experimental Dermatology
Matthias VockelJ M Brandner

Abstract

Somatostatin (SST) is a regulatory peptide hormone that acts through five different G protein-coupled receptors (SSTR1-5). Whereas expression of all five SSTR subtypes in epidermis has been shown, the biological relevance of the SST/SSTR system in the skin is completely unknown. We show here that SST is expressed in human skin and is present in a subset of Merkel cells and dendritic cells as well as in keratinocytes. We focused further on the somatostatin receptor subtype 3 (SSTR3) and its interacting protein MUPP1, as both were found to be localized at cellular junctions in epidermal keratinocytes. MUPP1 is a component of tight junctions (TJs); these cell-cell junctions contribute to barrier function of the paracellular pathway in cultured keratinocytes. We provide evidence that SSTR3 and MUPP1 interact in primary cultured human keratinocytes at high Ca(2+) conditions. Interestingly, SST, presumably via SSTR3/MUPP1, regulates TJ permeability in cultured keratinocytes. During long-term treatment of human keratinocytes, SST also affects the expression of distinct TJ proteins such as claudin-4. Our data are the first example of a peptide hormone regulating TJ functionality and composition in human keratinocytes, suggesting that c...Continue Reading

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Citations

Apr 5, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Zhang-Jin ZhangGrainne M McAlonan
Dec 3, 2013·Histochemistry and Cell Biology·Thomas TillingIngrid Moll
Jun 8, 2012·Annals of the New York Academy of Sciences·Nina Kirschner, Johanna M Brandner
Dec 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Iain S HaslamRalf Paus
Apr 4, 2015·Tissue Barriers·J M BrandnerA De Benedetto
Feb 11, 2015·Cell and Tissue Research·Johanna M Brandner, Jörg D Schulzke
Mar 2, 2019·Endocrinology·Courtney OlsenJeffrey A Frost
Aug 14, 2012·Experimental Dermatology·Anna CelliTheodora M Mauro
Feb 9, 2018·Tissue Barriers·Lorenza González-MariscalChristian Hernández-Guzmán
May 28, 2014·Experimental Dermatology·Ying XiaoIsaac Brownell
Mar 27, 2015·Molecular Pharmacology·Henry A Dunn, Stephen S G Ferguson

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