Somatostatin release from rat hypothalamus in vitro: effects of melatonin and serotonin

Endocrinology
S B RichardsonY Hirooka

Abstract

The release of immunoreactive somatostatin (SRIF) from explants of rat medial basal hypothalamus, which were maintained in culture for 24 h, was quantitated by a sensitive RIA. Validity of the specific SRIF assay has been previously established by chromatographic criteria using gel and high pressure systems and by the demonstration of immunological parallelism. After 24 h of culture in medium containing heat-inactivated fetal calf serum, hypothalamic fragments were incubated in serum-free medium, and the release of immunoreactive SRIF was quantitated. Melatonin at concentrations of 10(--8) and 10(--7) M stimulated SRIF release, and no significant increases were observed at concentrations of 10(--9) M or less or at concentrations of 10(--6) M or greater. Serotonin oxalate at concentrations of 10(--8)--10(--5) M significantly inhibited SRIF release. The serotonin antagonist cyproheptadine at a concentration of 10(--5)M had no effect on basal SRIF release but abolished the inhibitory effect of 10(--7) M serotonin. Finally, when hypothalami were incubated with melatonin and serotonin, each at 10(--7) M, SRIF release was unchanged compared to control values. The results suggest that the brain indoleamines, melatonin and serotonin, m...Continue Reading

Citations

May 3, 1990·European Journal of Pharmacology·M Toivonen, P T Männistö
Jan 1, 1991·General Pharmacology·J PopovaN Iavorska
Jul 1, 1991·Domestic Animal Endocrinology·G S SpencerJ J Bass
Sep 19, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Eleftherios PallisKyriaki Thermos
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S B RichardsonC S Hollander
May 2, 2014·Journal of Molecular Endocrinology·Tamar Eigler, Anat Ben-Shlomo
Aug 7, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Jin JinTsutomu Hashizume
Feb 7, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Jin JinTsutomu Hashizume
Jul 6, 2010·Animal Science Journal = Nihon Chikusan Gakkaihō·Etsuko KasuyaMadoka Sutoh
Nov 7, 2006·The Journal of Veterinary Medical Science·Etsuko KasuyaKoichi Hodate
Feb 26, 1982·Biochemical and Biophysical Research Communications·N Zisapel, M Laudon
Jun 1, 1983·The American Journal of Physiology·S B RichardsonC S Hollander

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