Some aspects of E. coli promoter evolution observed in a molecular evolution experiment.

Journal of Molecular Evolution
Shumo Liu, Albert Libchaber

Abstract

We devised a molecular evolution procedure to evolve E. coli promoter sequences and applied it to observe an arbitrary, nonfunctional sequence evolving into functional promoters. In the experiments, DNA sequence variations were generated with error-prone PCR and were inserted in the promoter region of the cat (chloramphenicol acetyl transferase) gene on a plasmid. Upon transforming the cells, functional promoters on the plasmid were selected according to the chloramphenicol resistance. Within a few cycles of mutation-selection, promoters emerged, and the sequences converged into a small number of groups. In the process, the extended minus 10 type of promoters emerged quickly, and small deletions were often involved in adjusting the length between the -35 and the -10 elements. Our results also suggest a possible selection for promoter stability against mutation.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Mar 15, 1977·European Journal of Biochemistry·P H SeeburgH Schaller
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D Pribnow
Aug 1, 1992·PCR Methods and Applications·R C Cadwell, G F Joyce
Jan 1, 1987·Methods in Enzymology·A R Oliphant, K Struhl
Aug 11, 1988·Nucleic Acids Research·A R Oliphant, K Struhl
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M S Horwitz, L A Loeb
Mar 11, 1987·Nucleic Acids Research·C B Harley, R P Reynolds
Jan 1, 1984·Annual Review of Genetics·O Raibaud, M Schwartz
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·S T EstremR L Gourse
Dec 12, 2001·Molecular Microbiology·T GaalR L Gourse
Mar 1, 1952·Journal of Bacteriology·J LEDERBERG, E M LEDERBERG
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mofang LiuSankar Adhya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
John Gerhart, Marc Kirschner
Proceedings of the National Academy of Sciences of the United States of America
H BrismarA Aperia
© 2022 Meta ULC. All rights reserved