Some autoantibodies to Ro/SS-A and La/SS-B are antiidiotypes to anti-double-stranded DNA

Arthritis and Rheumatism
W Zhang, M Reichlin

Abstract

To determine the relationship of anti-Ro/SS-A and anti-La/SS-B antibodies to anti-double- stranded DNA (anti-dsDNA) in sera from patients with systemic lupus erythematosus. Sera with anti-Ro/SS-A alone (n = 5) or those with anti-Ro/SS-A and anti-La/SS-B (n = 7) were absorbed with purified Ro/SS-A and La/SS-B, respectively. The absorbed sera were then tested for reactivity with MOLT-4 extract by Western blot and dsDNA by enzyme-linked immunosorbent assay (ELISA). With selected sera, anti-dsDNA was isolated on DNA cellulose columns and anti-Ro/SS-A and anti-La/SS-B were isolated on antigen-affinity columns. Reactivity between anti-dsDNA and autologous anti-Ro/SS-A or anti-La/SS-B, as well as inhibition by cognate antigens, was studied. After absorption, all sera showed reactivity with small nuclear RNP A and D bands in Western blots, and some showed reactivity with dsDNA by ELISA. Anti-dsDNA populations (n = 4) were purified on dsDNA cellulose columns. Anti-Ro/SS-A (n = 1) and anti-La/SS-B (n = 3) were affinity purified from the same sera as the anti-dsDNA. In all cases, anti-dsDNA bound autologous anti-Ro/SS-A and anti-La/SS-B much more strongly than it bound normal pooled IgG. Moreover, dsDNA, but not RNA, blocked these interac...Continue Reading

References

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Citations

Feb 23, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·Francisco Cabral-ArellanoRafael Herrera-Esparza
Jul 11, 2002·Oral Diseases·Roland JonssonPeter Szodoray
Apr 16, 2013·Biochemistry. Biokhimii︠a︡·V N BunevaG A Nevinsky
Apr 29, 2008·Arthritis and Rheumatism·Diane L KamenGary S Gilkeson
Dec 14, 2011·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Qingping YaoXiaofeng Wang

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