PMID: 7017011Mar 1, 1981Paper

Some biochemical properties of pemphigoid antigen bound to the surface of dissociated epidermal basal cells

The Journal of Investigative Dermatology
P M GrekinL A Diaz

Abstract

Bullous pemphigoid antigen (BP Ag) is a cell surface marker of epidermal basal cells. The functional role of this molecule is unknown. Epidermal cell suspensions obtained by trypsinization of skin show a population of epidermal basal cells with a polar rim of antigen as demonstrated by indirect immunofluorescence technique. This study shows that treatment of these cells suspensions with a variety of proteolytic and glycosidic enzymes failed to remove the antigen from these basal cells. BP Ag was also stable upon incubation with distilled water, Triton X-100, PBS, and 1 M NaCl. Treatment of epidermal basal cells with 2 N NaSCN, 1% periodic acid, and 4 M urea, as well as acidic pH or 56 degrees C temperature, abolished the reactivity of these cells with BP antibodies.

Citations

Jul 1, 1994·The British Journal of Dermatology·G KirtschigF Wojnarowska
Jul 23, 2004·Expert Opinion on Investigational Drugs·A Razzaque Ahmed
Jan 1, 1992·The British Journal of Dermatology·S E KellyS Fleming
Jul 1, 1984·The British Journal of Dermatology·M RegnierM Prunieras
Jan 1, 1987·Clinics in Dermatology·L A Diaz, G J Anhalt
Jan 1, 1982·The Journal of Investigative Dermatology·R A Briggaman
May 1, 1990·The Journal of Investigative Dermatology·L J MeyerJ J Zone
Feb 1, 1985·Journal of the American Academy of Dermatology·D T WoodleyM Prunieras

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bullous Pemphigoid

Bullous pemphigoid is a rare immune system disorder and skin condition that causes large, fluid-filled blisters. Bullous pemphigoid commonly affects older adults and appears in areas such as the lower abdomen, upper thighs or armpits. Discover the latest research on bullous pemphigoid here.